Western blot abreview for Anti-MMP9 antibody [EP1254]

Excellent
Abreviews
Application
Western blot
Sample
Rat Tissue lysate - whole (kidney cortex and outer medulla)
Gel Running Conditions
Reduced Denaturing (10-20% Criterion SDS-HCl)
Loading amount
30 µg
Specification
kidney cortex and outer medulla
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBST
Dilution
1/500

Secondary antibody

Dilution
1/5000
Name
Non-Abcam antibody was used: anti-rabbit IgG (HRP)
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL
Negative control
MMP9 knock out rat tissue
Exposure
20 second(s)
Bands
Specific: ~100 kDa Non-specific: 66, 50 , 40 kDa
Positive control
MMP9 wild type rat tissues
Username

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Submitted Oct 27 2017

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