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Products:Cardiovascular >> Angiogenesis >> Adhesion / ECM >> Matrix Metalloproteinases >> MMP
Overview
Product name
Anti-MMP9 antibody - Hinge region
See all MMP9 products (27) ...
Description
Rabbit polyclonal to MMP9 - Hinge region
Specificity
This antibody recognizes both native and reduced forms of MMP9. It does not cross react with other MMP family members (MMP1, MMP2, MMP3, etc).
Tested applications
WB, Sandwich ELISAmore details
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide based on the hinge region of the Human MMP9.
(Peptide available as ab41069.)
Positive control
WB: Human MMP9 and cell media from human chondrosarcoma.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cardiovascular >> Atherosclerosis >> Thrombosis >> Other
Cancer >> Tumor biomarkers >> Enzymes >> MMPs
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Metalloprotease >> MMPs
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> MMPs
Cancer >> Invasion/microenvironment >> Angiogenesis >> ECM enzymes >> MMPs
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> MMP
Cardiovascular >> Angiogenesis >> Adhesion / ECM >> Matrix Metalloproteinases >> MMP
Applications
Show applications key
Our Abpromise guarantee covers the use of ab38902 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use at an assay dependent dilution. Detects a band of approximately 92 kDa (predicted molecular weight: 78 kDa).
sELISA: Use a concentration of 0.5 µg/ml Can be paired for Sandwich ELISA with Mouse monoclonal [SB15c] to MMP9 (ab51203). (For sandwich ELISA, use this antibody as Detection at 0.5µg/ml with ab51203 as Capture.)
Target
Function
May play an essential role in local proteolysis of the extracellular matrix and in leukocyte migration. Could play a role in bone osteoclastic resorption. Cleaves KiSS1 at a Gly-
-Leu bond. Cleaves type IV and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments. Degrades fibronectin but not laminin or Pz-peptide.
Tissue specificity
Produced by normal alveolar macrophages and granulocytes.
Involvement in disease
Defects in MMP9 may be a cause of susceptibility to intervertebral disc disease (IDD) [MIM:603932]; also known as lumbar disk herniation (LDH). IDD is one of the most common musculo-skeletal disorders and the predominant cause of low-back pain and unilateral leg pain.
Defects in MMP9 are the cause of metaphyseal anadysplasia type 2 (MANDP2) [MIM:613073]. Metaphyseal anadysplasia consists of an abnormal bone development characterized by severe skeletal changes that, in contrast with the progressive course of most other skeletal dysplasias, resolve spontaneously with age. Clinical characteristics are evident from the first months of life and include slight shortness of stature and a mild varus deformity of the legs. Patients attain a normal stature in adolescence and show improvement or complete resolution of varus deformity of the legs and rhizomelic micromelia.
Sequence similarities
Belongs to the peptidase M10A family.
Contains 3 fibronectin type-II domains.
Contains 4 hemopexin-like domains.
Domain
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
Post-translational
modifications
Processing of the precursor yields different active forms of 64, 67 and 82 kDa. Sequentially processing by MMP3 yields the 82 kDa matrix metalloproteinase-9.
N- and O-glycosylated.
Cellular localization
Secreted > extracellular space > extracellular matrix.
Target information above from: UniProt accessionP14780
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- 82 kDa matrix metalloproteinase-9 antibody
- 92 kDa gelatinase antibody
- 92 kDa type IV collagenase antibody
see all
Database links
- Entrez Gene: 4318 Human
- Omim: 120361 Human
- SwissProt: P14780 Human
- Unigene: 297413 Human
Anti-MMP9 antibody - Hinge region images:
Sandwich ELISA - MMP9 antibody - Hinge region (ab38902)
Standard curve for MMP9 (Analyte: ab82955); dilution range 1pg/ml to 1µg/ml using Capture Antibody Mouse monoclonal [SB15c] to MMP9 (ab51203) at 5µg/ml and Detector Antibody Rabbit polyclonal to MMP9 - Hinge region (ab38902) at 0.5µg/ml.
Western blot - MMP9 antibody (ab38902)
All lanes : Anti-MMP9 antibody - Hinge region (ab38902)
Lane 1 : Human MMP9
Lane 2 : Cell media from human chondrosarcoma (untreated)
Predicted band size : 78 kDa
Observed band size : 92 kDa (why is the actual band size different from the predicted?)
When used against the reduced protein, ab38902 identifies bands at 92kDa and 88kDa (the pro form and active form respectively).
References for Anti-MMP9 antibody - Hinge region (ab38902)
ab38902 has not yet been referenced specifically in any publications.
Publishing research using ab38902? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"