Products:Cardiovascular >> Angiogenesis >> Adhesion / ECM >> Matrix Metalloproteinases >> MMP
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Publishing research using ab38898? Please let us know so that we can cite the reference in this datasheet
ab38898 has been referenced in 26 publications.
Publishing research using ab38898? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Anti-MMP9 antibody - Whole molecule (ab38898) + Human MMP9
Observed band size : 88,92 kDa (why is the actual band size different from the predicted?)
Ab38898 detects a band at 92 Kd (pro-form) and a band at 88 Kd (active form). Mouse MMP9 is slightly larger than human MMP9, and the antibody detects a band at about 105 Kd.
It is recommend to concentrate samples by Gelatin-agarose affinity chromatography prior to Western blot usage. A recommended starting concentration for Western blots is 1:1000 when using colorimetric substrates such as BCIP/NBT, and 1:5000 for chemiluminescent substrates. Higher concentration of antibody may be needed for non-human samples.
ab38898 at a 1/1000 dilution staining mouse heart tissue by Immunohistochemistry (Frozen sections). The tissue was removed from a mouse, rinsed in PBS and slowly frozen in supercooled isopentane. 14um sections were made using a cryostat. The sections were acetone fixed and blocked in 2% BSA prior to incubation with the MMP9 antibody. A Cy3 conjugated goat anti-rabbit antibody was used as the secondary antibody. In the image: red staining = MMP9, blue staining = nuclei, green = gelatinase activity.
All lanes : Anti-MMP9 antibody - Whole molecule (ab38898) at 1/1000 dilution
Lane 1 : Human lung tissue lysate at 100 µg with 10% Milk for 1 hour at room temperature
Lane 2 : Human lung tissue lysate at 100 µg with 10% Milk for 1 hour at room temperature
Lane 3 : MMP-9 KO mice tissue lysates with 10% Milk for 1 hour at room temperature
Lane 4 : MMP-9 KO mice tissue lysates with 10% Milk for 1 hour at room temperature
Secondary
HRP-conjugated donkey anti-rabbit polyclonal at 1/1000 dilution
developed using the ECL technique
Performed under reducing conditions.
Specific observed bands 95-100 kDa
This image is courtesy of an anonymous Abreview
ab38898 staining MMP9 in Mouse Pancreatic carcinoma tissue sections by IHC-P (formaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 1% BSA for 1 hour at room temperature. Antigen retrieval was by heat mediation in citric acid (pH6). Samples were incubated with primary antibody (1/100) in 1% Aurion BSA for 12 hours. An HRP-conjugated Donkey polyclonal to rabbit IgG (1/100) was used as secondary antibody.
This image is courtesy of an anonymous Abreview
18
Call 01223 696 000 or contact us