Anti-MMP9 antibody - Whole molecule (ab38898)

Thanks for reply.  First we did tried a bit on the suggestion tips. Second, as was suggested, the vial we received is likely a faulty vial, thereas, he has already issued us a free replacement with possible a different lot for try.  The suggestion is a general tip which we tested in our lab. We do not see any other need that we should ask again regarding this vial item.   We would thereas ask, that please send us a replacement vial as quickly as possibly, so that we may retry it again and hope it working.   Thanks If you have other questions please let me know.  

ANSWER:

This has kindly forward this message to me so that I might help.

I do see that the HCH2 antibody did arrive on 8 May. Have you had the opportunity to test this product? I do hope that this replacement has resolved the issue you were having.

Regarding the anti-MMP9 antibody; this was not included in the free of charge replacement as we were able to offer some advice which I had hoped would resolve the problem.

I recommended trying:"different blocking solution such as 3% BSA or using casein based blocking solutions such as our 10x blocking buffer, ab126587. While optimal concentrations of the primary and secondary antibodies will need to be determined in the lab, we recommend a starting concentration of 1:5000 for Western blots with chemiluminescent substrates. We also recommend a shorter secondary antibody incubation period."

Were you able to try these adjustments? Didthese suggestions help?

We guarantee all our products for TESTED species and applications as listed on the datasheet. The guarantee is valid for 6 months after a purchase. For more details about our Abpromise guarantee please check this link: http://www.abcam.com/abpromise.

I look forward to hearing from you as to how the new product and these suggestions worked for you. Please let me know if you have any questions.

Inquiry: Hi, I use your AB at mice at muscle (Longissmus) samples- for IHC Floro & for WB. The IHC is great and I can figure what I get but I'm not sure what I get at the WB- I get a lot of bands and don't know to say what is the MMP result from all the bands I get. (I use 10% gel and 5% skim milk as a blocking solution). I add my result (for WB & Pounceo Red) with the hope that you could help me to figure it. thanks,

ANSWER:

Thank you for contacting us and sending the image.

Without further details it is very difficult to make suggestions to improve the results from this antibody.

I will be very happy to help you, and for that, I would appreciate if you could please send me more protocol details. I send you a questionnaire that only take about 5 minutes to be completed, and could help us understand the results obtained. I would also appreciate if you could please send further details about the different lanes in the gel.

Once we receive the completed questionnaire, we will look at the protocol and see if there are any suggestions we can make that may improve the results. If no suggestions can be made to the protocol, and the antibody was purchased within the last 6 months, it is covered by our Abpromise guarantee, and therefore I can offer you a free of charge replacement or a credit note for it.

I look forward to receiving your reply. Please do not hesitate to contact us if you need any more advice or information.

Thanks for good support. We appreciate the suggestions of the antibody ab38898 and we will try to test again. Thank you for issuing replacement for ab65704, we would like to retest it again.

ANSWER:

You are very welcome.

Please let me know how the results turn out.

If you have any questions or there are other ways that Abcam may help you meet your research goals please do not hesitate to contact me.

Dear US Technical,

  We have another two antibodies that one showed too many bands, another negative in signals. See attached. We would like to hear of your advice or suggestions, hope the 2nd one would be replaced with a new product so that I may be able to try again.   Thanks for your time and support.

ANSWER:

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry to hear of these issues which you have been experiencing with ab38898 and ab65704 and wish to thank you for taking the time to send to me the protocols, troubleshooting steps and images regarding these.

For ab38898, our image on the datasheet shows that this product detects a band at 92 kDa (pro-form) and a band at 88 kDa (active form), but that there are other non specific present. To reduce the intensity of these, we recommend trying different blocking solution such as 3% BSA or using casein based blocking solutions such as our 10x blocking buffer, ab126587. While optimal concentrations of the primary and secondary antibodies will need to be determined in the lab, we recommend a starting concentration of 1:5000 for Western blots with chemiluminescent substrates. We also recommend a shorter secondary antibody incubation period.

Regarding ab65704, I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. I can from your protocol, troubleshooting and images that this problem is likely a faulty vial. As requested, I have issued a free of charge replacement of a new lot with the order number XXXXX.

To check the status of the order please contact our Customer Service team and reference this number.

Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

I wish you the best of luck with your research.

could you please let me know if Ab38898 recognise both the proform and ans also the activated for of the protein?Thanks in advance ind regards

ANSWER:

Thank you for contacting us.

This antibody should be detecting both the pro form and cleaved activate form of MMP9.

I hope this info will be helpful.

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