MMP9 protein (His tag) (ab82955)
Constituents: 50% Glycerol, PBS, pH 7.2
Our Abpromise guarantee covers the use of ab82955 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|SDS-PAGE||SDS-PAGE: Use at an assay dependent dilution.|
|WB||WB: Use at an assay dependent concentration. Ab82955 can be used as a WB positive control in conjunction with ab73734.|
- 82 kDa matrix metalloproteinase-992 kDa gelatinase92 kDa type IV collagenase
- CLG 4BCLG4BCollagenase Type 4 betaCollagenase type IV 92 KDEC 126.96.36.199Gelatinase 92 KDGelatinase BGelatinase betaGelatinaseBGELBMacrophage gelatinaseMANDP2Matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase)Matrix Metalloproteinase 9MMP 9MMP-9MMP9MMP9_HUMANType V collagenase
-Leu bond. Cleaves type IV and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments. Degrades fibronectin but not laminin or Pz-peptide.
Metaphyseal anadysplasia 2 (MANDP2) [MIM:613073]: A bone development disorder characterized by skeletal anomalies that resolve spontaneously with age. Clinical characteristics are evident from the first months of life and include slight shortness of stature and a mild varus deformity of the legs. Patients attain a normal stature in adolescence and show improvement or complete resolution of varus deformity of the legs and rhizomelic micromelia. Note=The disease is caused by mutations affecting the gene represented in this entry.
Contains 3 fibronectin type-II domains.
Contains 4 hemopexin-like domains.
modificationsProcessing of the precursor yields different active forms of 64, 67 and 82 kDa. Sequentially processing by MMP3 yields the 82 kDa matrix metalloproteinase-9.
N- and O-glycosylated.
MMP9 protein (His tag) images
Standard curve for MMP9 (Analyte: ab82955); dilution range 1pg/ml to 1µg/ml using Capture Antibody Mouse monoclonal [SB15c] to MMP9 (ab51203) at 5µg/ml and Detector Antibody Rabbit polyclonal to MMP9 - Hinge region (ab38902) at 0.5µg/ml.
ab82955 on SDS-PAGE.
Anti-MMP9 antibody (ab73734) at 1 µg +
MMP9 protein (His tag) (ab82955) at 0.1 µg
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Exposure time : 2 minutes
References for MMP9 protein (His tag) (ab82955)
ab82955 has not yet been referenced specifically in any publications.