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Overview

  • Product nameMMP9 protein (His tag)See all MMP9 proteins and peptides ...
  • Protein descriptionRecombinant full length Human MMP9 protein (proform without signal sequence) with an N-terminal 6xHis tag; amino acids 20-707 (688aa), 77kDa. Swiss ID P14780.
  • Expression hostE. coli

    • Properties

  • Purity> 95 % by SDS-PAGE
  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 50% Glycerol, PBS, pH 7.2
  • Concentration information loading...
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab82955 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    SDS-PAGE SDS-PAGE: Use at an assay dependent dilution.
    WB WB: Use at an assay dependent concentration. Ab82955 can be used as a WB positive control in conjunction with ab73734.

    Protein info

    • Alternative names
        82 kDa matrix metalloproteinase-992 kDa gelatinase92 kDa type IV collagenase
        CLG 4BCLG4BCollagenase Type 4 betaCollagenase type IV 92 KDEC 3.4.24.35Gelatinase 92 KDGelatinase BGelatinase betaGelatinaseBGELBMacrophage gelatinaseMANDP2Matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase)Matrix Metalloproteinase 9MMP 9MMP-9MMP9MMP9_HUMANType V collagenase
      see all
  • FunctionMay play an essential role in local proteolysis of the extracellular matrix and in leukocyte migration. Could play a role in bone osteoclastic resorption. Cleaves KiSS1 at a Gly-
    -Leu bond. Cleaves type IV and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments. Degrades fibronectin but not laminin or Pz-peptide.
  • Tissue specificityProduced by normal alveolar macrophages and granulocytes.
  • Involvement in diseaseIntervertebral disc disease (IDD) [MIM:603932]: A common musculo-skeletal disorder caused by degeneration of intervertebral disks of the lumbar spine. It results in low-back pain and unilateral leg pain. Note=Disease susceptibility is associated with variations affecting the gene represented in this entry.
    Metaphyseal anadysplasia 2 (MANDP2) [MIM:613073]: A bone development disorder characterized by skeletal anomalies that resolve spontaneously with age. Clinical characteristics are evident from the first months of life and include slight shortness of stature and a mild varus deformity of the legs. Patients attain a normal stature in adolescence and show improvement or complete resolution of varus deformity of the legs and rhizomelic micromelia. Note=The disease is caused by mutations affecting the gene represented in this entry.
  • Sequence similaritiesBelongs to the peptidase M10A family.
    Contains 3 fibronectin type-II domains.
    Contains 4 hemopexin-like domains.
  • DomainThe conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
  • Post-translational
    modifications    Processing of the precursor yields different active forms of 64, 67 and 82 kDa. Sequentially processing by MMP3 yields the 82 kDa matrix metalloproteinase-9.
    N- and O-glycosylated.
  • Cellular localizationSecreted > extracellular space > extracellular matrix.

    • Target information above from: UniProt accession P14780 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    MMP9 protein (His tag) images

    • Sandwich ELISA - MMP9 protein (His tag) (ab82955)
      Sandwich ELISA - MMP9 protein (His tag) (ab82955)
      Standard curve for MMP9 (Analyte: ab82955); dilution range 1pg/ml to 1µg/ml using Capture Antibody Mouse monoclonal [SB15c] to MMP9 (ab51203) at 5µg/ml and Detector Antibody Rabbit polyclonal to MMP9 - Hinge region (ab38902) at 0.5µg/ml.
    • SDS-PAGE - MMP9 protein (His tag) (ab82955)
      SDS-PAGE - MMP9 protein (His tag) (ab82955)
      ab82955 on SDS-PAGE.
    • Western blot
      Western blot
      Anti-MMP9 antibody (ab73734) at 1 µg + MMP9 protein (His tag) (ab82955) at 0.1 µg

      Secondary
      Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
      developed using the ECL technique

      Performed under reducing conditions.

      Exposure time : 2 minutes

    References for MMP9 protein (His tag) (ab82955)

    ab82955 has not yet been referenced specifically in any publications.

    Publishing research using ab82955 ? Please let us know so that we can cite the reference in this datasheet

    Product Wall

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    Dear technical team,

    Our customer needs MMP9 protein active and inactive form.

    I have searched MMP9 but couldn't get information about active and inactive form.

    Let me know, please.


    Best regards,
    Youn...

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    Thank you once again for your enquiries regarding our MMP9 proteins.

    To follow up, I now have further information regarding the following proteins:

    ab39308 MMP9 protein (Human)
    ab39309 MMP9 protein (Mouse)
    ab41067 MMP9 pept...

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    Thank you for your answer and apologies for my late reply! I have been on vacation, and since yesterday trying to answer all the mail in my mailbox...

    Yes, it seems like there is some batch-related problem with MUC1, thank you for following tha...

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    Thank you very much for your email!

    With some delay - I wish you also all the best for 2012, especially also for your new position! I hope you are still at Glykos currently. Please let me know else whether I should address myself directly to you...

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    I promised you some information about the antigen proteins before Christmas, and I could just make it, although I only had time to run an SDS-PAGE gel. So, I have attached here a picture of the gel. I also included in the gel the new CEA protein that I or...

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    Thank you very much for the follow up. I am embarassed and very sorry for what has happened. It is most unfortunate that the new MMP9 protein also seems to have vanished. I will follow this up with the respective laboratory immediately. Indeed, fo...

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