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Read our guarantee »Overview
Product name
MOUSE IgG1 antibody [MOPC-21] (FITC)
Description
Mouse monoclonal [MOPC-21] MOUSE IgG1 (FITC)
Conjugation
FITC
Conjugation notes
Conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC.
Specificity
ab106163 (clone MOPC-21) has unknown specificity and was chosen as an isotype control after screening on variety of resting, activated, live and fixed Rat and Human tissues.
Tested applications
WB, IP, IHC-P, IHC-Fr, Flow Cytmore details
Cross reactivity
General notes
ab106163 is intended as isotype control for flow cytometry analysis to establish the amount of non-specific antibody binding. For your particular experiment, use the same concentration of this isotype control antibody as the recommended working concentration of the antigen-specific antibody. Also, when working with prediluted antibodies, dilute the isotype control to the same concentration as is the concentration of the antigen-specific antibody in the prediluted antibody solution you are using. If under particular experimental conditions the background signal of the isotype control is too high (usually when working concentrations of used antibodies are above 10 µg per ml of incubation mixture), change the conditions of your experiment to reduce the background.
Properties
Form
Liquid
Storage instructions
Store at +4°C in the dark.
Storage buffer
Preservative: 15mM Sodium Azide
Constituents: PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Protein G purified
Purification notes
> 95% (by SDS-PAGE)
Clonality
Monoclonal
Clone number
MOPC-21
Isotype
IgG1
Light chain type
kappa
Research areas
Immunology >> Immunoglobulins >> Heavy Chain >> IgG
Relevance
Immunoglobulin G (IgG), is one of the most abundant protein in human serum with normal levels between 8-17 mg/ml in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B-lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.
Cellular localization
Secreted
Alternative names
- Constant region of heavy chain of IgG1
- FCGBP
- FCGR1A
see all
Cellular localization
Secreted
Applications
Show applications key
Our Abpromise guarantee covers the use of ab106163 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use at an assay dependent dilution.
IP: Use at an assay dependent dilution.
IHC-P: Use at an assay dependent dilution.
IHC-Fr: Use at an assay dependent dilution.
Flow Cyt: Use at an assay dependent dilution.
References for MOUSE IgG1 antibody [MOPC-21] (FITC) (ab106163)
This product has been referenced in:
- Smed-Sörensen A et al. IgG regulates the CD1 expression profile and lipid antigen-presenting function in human dendritic cells via FcgammaRIIa. Blood 111:5037-46 (2008).Read more (PubMed: 18337560) »
- Yates J et al. The maintenance of human CD4+ CD25+ regulatory T cell function: IL-2, IL-4, IL-7 and IL-15 preserve optimal suppressive potency in vitro. Int Immunol 19:785-99 (2007).Read more (PubMed: 17545278) »
See all 4 publications for this product
Publishing research using ab106163? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"