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Overview

  • Product nameAnti-MPP5 antibodySee all MPP5 primary antibodies ...
  • Description
    Rabbit polyclonal to MPP5
  • Tested applicationsWB more details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Chicken, Cow, Dog
  • Immunogen

    Synthetic peptide corresponding to a region within the N terminal amino acids 36-85 (AVDCPGDLGTRMMPIRRSAQLERIRQQQEDMRRRREEEG KKQELDLNSSM) of Human MPP5 (NP_071919)

  • Positive controlJurkat cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab83891 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB
  • Application notesWB: Use at a concentration of 1 µg/ml. Detects a band of approximately 77 kDa (predicted molecular weight: 77 kDa).
    ELISA titre using peptide based assay: 1/312500.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Target

    • FunctionMay play a role in tight junctions biogenesis and in the establishment of cell polarity in epithelial cells. May modulate SC6A1/GAT1-mediated GABA uptake by stabilizing the transporter. Required for localization of EZR to the apical membrane of parietal cells and may play a role in the dynamic remodeling of the apical cytoskeleton.
    • Tissue specificityExpressed in the retina (at protein level).
    • Sequence similaritiesBelongs to the MAGUK family.
      Contains 1 guanylate kinase-like domain.
      Contains 2 L27 domains.
      Contains 1 PDZ (DHR) domain.
      Contains 1 SH3 domain.
    • DomainThe L27 domain 1 functions in targeting to the tight junctions by binding to INADL.
      The PDZ domain binds to the C-terminus of SC6A1.
    • Cellular localizationCell membrane. Endomembrane system. Cell junction > tight junction. Localized to the tight junctions of epithelial cells and a subset of intracellular vesicles. In the retina, detected at the outer limiting membrane (OLM), apical to the adherens junction (AJ), where it colocalizes with CRB1. Colocalizes with MPP1 in the retina at the outer limiting membrane (OLM). Localized to the Purkinje cell body and axon.

      • Target information above from: UniProt accession Q8N3R9 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links
      see all
    • Alternative names
        FLJ12615 antibodyMAGUK p55 subfamily member 5 antibodyMembrane protein palmitoylated 5 antibody
        MPP 5 antibodyMPP5 antibodyMPP5_HUMAN antibodyPALS 1 antibodyPALS1 antibodyProtein associated with Lin-7 antibodyStardust (Drosophila) antibodyStardust antibody
      see all

    Anti-MPP5 antibody images

    • Western blot - MPP5 antibody (ab83891)
      Western blot - MPP5 antibody (ab83891)
      Anti-MPP5 antibody (ab83891) at 1 µg/ml + Jurkat cell lysate at 10 µg

      Secondary
      HRP conjugated anti-Rabbit IgG at 1/50000 dilution

      Predicted band size : 77 kDa
      Observed band size : 40,77 kDa (why is the actual band size different from the predicted?)

    References for Anti-MPP5 antibody (ab83891)

    ab83891 has not yet been referenced specifically in any publications.

    Publishing research using ab83891 ? Please let us know so that we can cite the reference in this datasheet

    Product Wall

    Submit an Abreview for ab83891 Submit a Question for ab83891

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    10
    Votes: Highest First

    Dear Tineke/Abcam,

    We purchased the following Abcam antibodies from Sapphire Biosciences as one single order which were shipped to us as a single lot:

    1 AB68466 Rabbit polyclonal to PSAP

    1 AB36970 Rabbit polyclonal to Scav...

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    Thank you for your reply and all the additional information. This makes the problems you have been experiencing much clearer to understand. It is clear that you have spent a lot of time in trying to optimise these antibodies and I am sorry that they are n...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"