Recombinant Anti-MPZL antibody [EPR9229] (ab151541)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9229] to MPZL
- Suitable for: WB, ICC/IF
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-MPZL antibody [EPR9229]
See all MPZL primary antibodies -
Description
Rabbit monoclonal [EPR9229] to MPZL -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details
Unsuitable for: Flow Cyt,IHC-P or IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human MPZL aa 200-300 (intracellular). The exact sequence is proprietary.
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Positive control
- WB: 293T, HeLa and HepG2 whole cell lysate (ab7900). ICC/IF: HepG2 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR9229 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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KO cell lines
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KO cell lysates
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab151541 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 29 kDa.
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ICC/IF |
1/250 - 1/500.
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Notes |
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WB
1/1000 - 1/10000. Predicted molecular weight: 29 kDa. |
ICC/IF
1/250 - 1/500. |
Target
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Relevance
Cell surface receptor, which is involved in signal transduction processes. Is a major receptor for concanavalin A (ConA) and is involved in cellular signaling induced by ConA, which probably includes Src family tyrosine protein kinases. Isoform 3 seems to block tyrosine phosphorylation of MPZL1 induced by ConA. Isoform 1, unlike isoforms 2 and 3, may be involved in regulation of integrin mediated cell motility. -
Cellular localization
Membrane; single pass type I membrane protein (Potential) -
Database links
- Entrez Gene: 9019 Human
- GenBank: BC007881 Human
- Omim: 604376 Human
- SwissProt: O95297 Human
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Alternative names
- immunoglobulin family transmembrane protein antibody
- MPZL1 antibody
- MPZL1b antibody
see all
Images
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All lanes : Anti-MPZL antibody [EPR9229] (ab151541) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : MPZL1 knockout HeLa cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : 293T cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 29 kDa
Observed band size: 37 kDa why is the actual band size different from the predicted?Lanes 1-4: Merged signal (red and green). Green - ab151541 observed at 37 kDa. Red - loading control ab7291 observed at 50 kDa.
ab151541 Anti-MPZL antibody [EPR9229] was shown to specifically react with MPZL in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265332 (knockout cell lysate ab258053) was used. Wild-type and MPZL knockout samples were subjected to SDS-PAGE. ab151541 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated at room temperature for 2.5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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Immunofluorescent analysis of HepG2 cells labeling MPZL with ab151541 at 1/250 dilution.
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All lanes : Anti-MPZL antibody [EPR9229] (ab151541) at 1/1000 dilution
Lane 1 : 293T cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : HepG2 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 29 kDa
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab151541 has been referenced in 2 publications.
- Liu X et al. MPZL1 is highly expressed in advanced gallbladder carcinoma and promotes the aggressive behavior of human gallbladder carcinoma GBC-SD cells. Mol Med Rep 20:2725-2733 (2019). PubMed: 31322261
- Liu Z et al. Specific marker expression and cell state of Schwann cells during culture in vitro. PLoS One 10:e0123278 (2015). PubMed: 25859851