Anti-MRCL3 antibody (ab131530)
- Product nameAnti-MRCL3 antibodySee all MRCL3 primary antibodies ...
- DescriptionRabbit polyclonal to MRCL3
- Specificityab131530 detects endogenous levels of total MRCL3.
- Tested applicationsWB, ICC/IF more details
- Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide conjugated to KLH, surrounding amino acids 16-20 (A-T-S-N-V) of Human MRCL3 (NP_006462.1).
- Positive controlMethanol-fixed HeLa cells; K562 cell extracts.
- Storage instructionsStore at -20°C. Stable for 12 months at -20°C
- Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.88% Sodium chloride
Note: PBS without Mg2+ and Ca2+
- Concentration information loading...
- PurityImmunogen affinity purified
- Purification notesab131530 was purified by affinity chromatography using epitope specific peptide.
- Clonality Polyclonal
- Research Areas
Our Abpromise guarantee covers the use of ab131530 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||WB: 1/500 - 1/1000. Predicted molecular weight: 20 kDa.|
|ICC/IF||ICC/IF: 1/100 - 1/200.|
- FunctionMyosin regulatory subunit that plays an important role in regulation of both smooth muscle and nonmuscle cell contractile activity via its phosphorylation. Implicated in cytokinesis, receptor capping, and cell locomotion.
- Sequence similaritiesContains 3 EF-hand domains.
modificationsPhosphorylation increases the actin-activated myosin ATPase activity and thereby regulates the contractile activity. It is required to generate the driving force in the migration of the cells but not necessary for localization of myosin-2 at the leading edge.
- ML12A_HUMAN antibodyMLC 2B antibodyMLC-2B antibody
- MLCB antibodyMYL12A antibodyMyosin regulatory light chain 12A antibodyMyosin regulatory light chain 2 antibodyMyosin regulatory light chain 2, nonsarcomeric antibodyMyosin regulatory light chain MRCL3 antibodyMyosin regulatory light chain MRLC3 antibodyMyosin RLC antibodyMyosin, light polypeptide, regulatory, non sarcomeric (20kD) antibodynonsarcomeric antibodyRLC antibody
Anti-MRCL3 antibody images
All lanes : Anti-MRCL3 antibody (ab131530) at 1/500 dilution
Lane 1 : K562 cell extract with blocking peptide
Lane 2 : K562 cell extract
Predicted band size : 20 kDa
Immunofluorescent analysis of methanol-fixed HeLa cells labelling MRCL3 with ab131530 at 1/100 dilution.
References for Anti-MRCL3 antibody (ab131530)
ab131530 has not yet been referenced specifically in any publications.