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Overview

  • Product nameAnti-MRCL3 antibodySee all MRCL3 primary antibodies ...
  • Description
    Rabbit polyclonal to MRCL3
  • Specificityab131530 detects endogenous levels of total MRCL3.
  • Tested applicationsWB, ICC/IF more details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide conjugated to KLH, surrounding amino acids 16-20 (A-T-S-N-V) of Human MRCL3 (NP_006462.1).

  • Positive controlMethanol-fixed HeLa cells; K562 cell extracts.

Properties

  • FormLiquid
  • Storage instructionsStore at -20°C. Stable for 12 months at -20°C
  • Storage bufferpH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 49% PBS, 50% Glycerol, 0.88% Sodium chloride
    Note: PBS without Mg2+ and Ca2+
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesab131530 was purified by affinity chromatography using epitope specific peptide.
  • Clonality Polyclonal
  • IsotypeIgG
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab131530 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB WB: 1/500 - 1/1000. Predicted molecular weight: 20 kDa.
ICC/IF ICC/IF: 1/100 - 1/200.

Target

  • FunctionMyosin regulatory subunit that plays an important role in regulation of both smooth muscle and nonmuscle cell contractile activity via its phosphorylation. Implicated in cytokinesis, receptor capping, and cell locomotion.
  • Sequence similaritiesContains 3 EF-hand domains.
  • Post-translational
    modifications    Phosphorylation increases the actin-activated myosin ATPase activity and thereby regulates the contractile activity. It is required to generate the driving force in the migration of the cells but not necessary for localization of myosin-2 at the leading edge.

    • Target information above from: UniProt accession P19105 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
    • Alternative names
        ML12A_HUMAN antibodyMLC 2B antibodyMLC-2B antibody
        MLCB antibodyMYL12A antibodyMyosin regulatory light chain 12A antibodyMyosin regulatory light chain 2 antibodyMyosin regulatory light chain 2, nonsarcomeric antibodyMyosin regulatory light chain MRCL3 antibodyMyosin regulatory light chain MRLC3 antibodyMyosin RLC antibodyMyosin, light polypeptide, regulatory, non sarcomeric (20kD) antibodynonsarcomeric antibodyRLC antibody
      see all

    Anti-MRCL3 antibody images

    • Western blot - Anti-MRCL3 antibody (ab131530)
      Western blot - Anti-MRCL3 antibody (ab131530)
      All lanes : Anti-MRCL3 antibody (ab131530) at 1/500 dilution

      Lane 1 : K562 cell extract with blocking peptide
      Lane 2 : K562 cell extract


      Predicted band size : 20 kDa
    • Immunocytochemistry/ Immunofluorescence - Anti-MRCL3 antibody (ab131530)
      Immunocytochemistry/ Immunofluorescence - Anti-MRCL3 antibody (ab131530)
      Immunofluorescent analysis of methanol-fixed HeLa cells labelling MRCL3 with ab131530 at 1/100 dilution.

    References for Anti-MRCL3 antibody (ab131530)

    ab131530 has not yet been referenced specifically in any publications.

    Publishing research using ab131530 ? Please let us know so that we can cite the reference in this datasheet

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"