Anti-MRP1 antibody (ab99531)

Overview

• Product nameAnti-MRP1 antibodySee all MRP1 primary antibodies ...
• Description
  Rabbit polyclonal to MRP1
• Tested applicationsWB, ICC/IF more details
• Species reactivity
  Reacts with: Human
  Predicted to work with: Dog, Monkey
• Immunogen

  Synthetic peptide conjugated to KLH derived from within residues 900 - 1000 of Human MRP1.

  Read Abcam's proprietary immunogen policy

• Positive controlThis antibody gave a positive signal in A549 whole cell lysate.

Properties

• FormLiquid
• Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferpH: 7.40
  Preservative: 0.02% Sodium azide
  Constituent: PBS
  Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
• Concentration information loading...
• PurityImmunogen affinity purified
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Metabolism
  • Types of disease
  • Cancer

  • Metabolism
  • Types of disease
  • Heart disease

  • Metabolism
  • Pathways and Processes
  • Metabolic signaling pathways
  • Drug metabolism

  • Metabolism
  • Pathways and Processes
  • Endocrine metabolism
  • Hormone biosynthesis

  • Cancer
  • Cancer Metabolism
  • Metabolic signaling pathway
  • Hormone biosynthesis

  • Cancer
  • Drug resistance
  • MRP-related proteins

  • Signal Transduction
  • Metabolism
  • Drug metabolism

  • Signal Transduction
  • Metabolism
  • Plasma Membrane
  • Channels

  • Cardiovascular
  • Blood
  • Blood Pressure regulation

  • Cardiovascular
  • Atherosclerosis
  • Hypertension
  • Sodium/ion transport

Applications

Target

• FunctionMediates export of organic anions and drugs from the cytoplasm. Mediates ATP-dependent transport of glutathione and glutathione conjugates, leukotriene C4, estradiol-17-beta-o-glucuronide, methotrexate, antiviral drugs and other xenobiotics. Confers resistance to anticancer drugs. Hydrolyzes ATP with low efficiency.
• Tissue specificityLung, testis and peripheral blood mononuclear cells.
• Sequence similaritiesBelongs to the ABC transporter superfamily. ABCC family. Conjugate transporter (TC 3.A.1.208) subfamily.
  Contains 2 ABC transmembrane type-1 domains.
  Contains 2 ABC transporter domains.
• Cellular localizationCell membrane.

Target information above from: UniProt accession P33527 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 403453 Dog
  • Entrez Gene: 4363 Human
  • Omim: 158343 Human
  • SwissProt: Q6UR05 Dog
  • SwissProt: P33527 Human
  • Unigene: 391464 Human

• Alternative names
  ABC 29 antibodyABC29 antibodyABCC 1 antibody

  ABCC antibodyABCC1 antibodyATP binding cassette sub family C (CFTR/MRP) member 1 antibodyATP binding cassette sub-family C member 1 antibodyATP-binding cassette sub-family C member 1 antibodyDKFZp686N04233 antibodyDKFZp781G125 antibodyGS X antibodyGSX antibodyLeukotriene C(4) transporter antibodyLTC4 transporter antibodyMRP 1 antibodyMRP antibodyMRP1_HUMAN antibodyMultidrug resistance associated protein 1 antibodyMultidrug resistance protein antibodyMultidrug resistance-associated protein 1 antibodyMultiple drug resistance associated protein antibodyMultiple drug resistance protein 1 antibody

  see all

Anti-MRP1 antibody images

• 

  Western blot - MRP1 antibody (ab99531)
  Anti-MRP1 antibody (ab99531) at 1 µg/ml + A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell Lysate at 10 µg
  
  Secondary
  Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
  developed using the ECL technique
  
  Performed under reducing conditions.
  
  Predicted band size : 171 kDa
  
  
  Exposure time : 8 minutes
• 

  Immunocytochemistry/ Immunofluorescence - MRP1 antibody (ab99531)
  ICC/IF image of ab99531 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab99531, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG(H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.