![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - MRP2 antibody [M2 III-6] (ab3373) image](#ab3373_1b.jpg)
![Western blot - MRP2 antibody [M2 III-6] (ab3373) image](#MRP2-Primary-antibodies-ab3373-4.jpg)
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Products:Signal Transduction >> Metabolism >> Plasma Membrane >> Channels
Overview
Product name
Anti-MRP2 antibody [M2 III-6]
See all MRP2 products (6) ...
Description
Mouse monoclonal [M2 III-6] to MRP2
Specificity
This antibody detects MRP 2. It does not cross-react with human MDR 1, MRP 1, MRP 3 or MRP 5 gene products.
Tested applications
Flow Cyt, IP, ICC, IHC-P, IHC-Fr, WBmore details
Cross reactivity
Reacts with
Rat, Human
Immunogen
Bacterial fusion protein of human MRP 2 containing the carboxy-terminal region (residues 1339-1541).
Epitope
This antibody reacts with an internal epitope of MRP 2.
Properties
Form
Liquid
Storage instructions
Store at +4°C.
Storage buffer
PBS with 1% BSA and 0.1% sodium azide
Concentration
Concentration information loading...
Clonality
Monoclonal
Clone number
M2 III-6
Isotype
IgG2a
Research areas
Cancer >> Drug resistance >> MRP-related proteins
Signal Transduction >> Metabolism >> Plasma Membrane >> Channels
Applications
Show applications key
Our Abpromise guarantee covers the use of ab3373 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Flow Cyt: Use at an assay dependent dilution. (PubMed: 18929567)
IP: Use at an assay dependent dilution. (PubMed: 16687572)
ICC: Use at an assay dependent dilution.
IHC-P: 1/200(See Abreview. Abreview indicates that several methods of antigen retreival are suitable for this antibody, but heat mediated Ag retrieval in citrate buffer gave optimal results.)
IHC-Fr: Use at an assay dependent dilution.
WB: 1/20 - 1/50.Predicted molecular weight: 174 kDa.(See Scheffer GL et al. 2000.)
Target
Function
Mediates hepatobiliary excretion of numerous organic anions. May function as a cellular cisplatin transporter.
Tissue specificity
Found on the apical membrane of polarized cells in liver, kidney and intestine. The highest expression is found in liver.
Involvement in disease
Defects in ABCC2 are the cause of Dubin-Johnson syndrome (DJS) [MIM:237500]. DJS is an autosomal recessive disorder characterized by conjugated hyperbilirubinemia, an increase in the urinary excretion of coproporphyrin isomer I, deposition of melanin-like pigment in hepatocytes, and prolonged retention of sulfobromophthalein, but otherwise normal liver function.
Sequence similarities
Belongs to the ABC transporter superfamily. ABCC family. Conjugate transporter (TC 3.A.1.208) subfamily.
Contains 2 ABC transmembrane type-1 domains.
Contains 2 ABC transporter domains.
Cellular localization
Membrane.
Target information above from: UniProt accessionQ92887
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- ABC30 antibody
- ABCC2 antibody
- ATP binding cassette sub family C (CFTR/MRP) member 2 antibody
see all
Database links
- Entrez Gene: 1244 Human
- Entrez Gene: 25303 Rat
- Omim: 601107 Human
- SwissProt: Q92887 Human
- SwissProt: Q63120 Rat
- Unigene: 193852 Human
- Unigene: 368243 Human
- Unigene: 10265 Rat
Anti-MRP2 antibody [M2 III-6] images:
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - MRP2 antibody [M2 III-6] (ab3373)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - MRP2 antibody [M2 III-6] (ab3373)](/ps/datasheet/Images/3/ab3373/ab3373_1b.jpg)
ab3373 at 1/200 staining rat liver tissue sections by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). The tissue was formaldehyde fixed and then heat mediated antigen retrieval was performed using citrate buffer. The antibody was incubated with the tissue for 1 hour. An HRP conjugated goat anti-mouse antibody was used as the secondary.
This image is courtesy of an Abreview submitted by Mr Rebecca Somers
Western blot - MRP2 antibody [M2 III-6] (ab3373)
![Western blot - MRP2 antibody [M2 III-6] (ab3373)](/ps/datasheet/images/3/ab3373/MRP2-Primary-antibodies-ab3373-4.jpg)
Anti-MRP2 antibody [M2 III-6] (ab3373) at 1 µg/ml + Liver (Rat) Tissue Lysate at 10 µg
Secondary
Goat polyclonal Secondary Antibody to Mouse IgG - H&L (HRP), pre-adsorbed (ab97040) at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 174 kDa
Observed band size : 171 kDa (why is the actual band size different from the predicted?)
Additional bands at : 31 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 20 minutes
References for Anti-MRP2 antibody [M2 III-6] (ab3373)
This product has been referenced in:
- Leitch Set al. Vesicular distribution of Secretory Pathway Ca²+-ATPase isoform 1 and a role in manganese detoxification in liver-derived polarized cells. Biometals 24:159-70 (2011). ICC/IF; Human.Read more (PubMed: 20981470) »
- Petrovic V & Piquette-Miller M Impact of polyinosinic/polycytidylic acid on placental and hepatobiliary drug transporters in pregnant rats. Drug Metab Dispos 38:1760-6 (2010).Read more (PubMed: 20610559) »
See all 8 publications for this product
Publishing research using ab3373? Please let us know so that we can cite the reference in this datasheet
![MRP2 antibody [M2 III-6] for IHC-P in Rat (3373)](/ps/Reviews/Images/ab6359_24498.jpg)
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"