You have changed your country from to
. Please be aware that this will change the currency in the purchasing process.
Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »This product is covered by the Abpromise guarantee. Our scientific support team are available to answer any questions or queries - fill out an inquiry form for ab15603 for help.
Alternatively, you can search the previous enquiries about this product to see if your query has already been answered.
|
|||||||||
 | |||||||||
|
|||||||||
I have used your M2III-5 antibody to examine the expression of mouse Mrp2 protein. Your product info sheet stated that M2III-5 can cross-react with rat, mouse and human Mrp2. Strangely, on our western blot a 170KD band was shown in both the wild type and the Mrp2 knockout samples. Honestly, I would think that band is the Mrp2 band if I did not have the Mrp2 KO sample as a negative control. We have work with M2III-6 a lot in our research, M2III-6 work beautifully in the WT and Mrp2-/- rats. From literature, Scheffer GL et al developed the clone for M2III-5 antibody and in their paper "M2III-5 was shown NO cross-reactivity to the mouse Mrp2" (table 1 of the paper, Cancer Research 2000, 60:5269-77). Also from the paper, M2III-5 and M2III-6 recognize the same aa sequence (1336-1541). M2III-6 has no cross-reactivity with mouse Mrp2 either although both M2III-5 and 6 can react with rat Mrp2 very well. I wonder if there is any difference between your M2III-5 antibody and the authors'. From my conversation with one of your tech support person this morning, you use the same clone as the authors. I personally think they should have the same reactivity. Could you please explain me why your antibody has different reactivity from author's? Is there any possibility that your M2III-5 can recognize a band other than Mrp2 in the mouse sample? Have you confirmed the specificity of your M2III-5 in mouse samples?
P.S. attachment of M2III-5 results with moue liver sample.
|
|||||||||
ANSWER: |
Thank you for your feedback regarding this product. As a result of the information that you have sent to me and references sourced via “Pub Med” I have currently removed cross reactivity with from our product data base. I would like to thank you for the information that you have forwarded and would be more than willing to offer either a credit note to your account/ refund. Could you confirm for me the order details cited below and I will arrange a credit note to be issued? Your PO or our Abcam order number The name of your purchasing agent and the shipping address. I look forward to hearing from you soon.
|
||||||||
|
|||||||||
| |||||||||
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"