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Anti-MRP2 antibody [M2III-5] (ab15603)

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Overview

Product name

Anti-MRP2 antibody [M2III-5]
See all MRP2 products (6) ...

Description

Mouse monoclonal [M2III-5] to MRP2

Specificity

This antibody does not cross-react with the human MDR1 P-gp, MRP1, MRP3, or MRP5 gene products.

Tested applications

WB, Flow Cyt, ICC, IHC-P, IHC-Frmore details

Cross reactivity

Reacts with

Rat, Human

Does not react with

Mouse

Immunogen

This antibody was raised against a fusion protein of the bacterial maltose binding protein and rat MRP2, containing the 202-amino acid C terminal end of the transporter protein.

Epitope

ab15603 reacts with an internal epitope of MRP2; a 190-200 kD transmembrane protein earlier known as the canalicular multi-organic anion transporter cMOAT, absent in patients with the Dubin-Johnson syndrome.

Properties

Form

Liquid

Storage instructions

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 0.1% Sodium Azide
Constituents: 0.7% BSA, Tissue culture supernatant

Purity

Tissue culture supernatant

Clonality

Monoclonal

Clone number

M2III-5

Isotype

IgG2b

Research areas

Cancer >> Drug resistance >> MRP-related proteins
Signal Transduction >> Metabolism >> Plasma Membrane >> Channels

Applications

Show applications key

Our Abpromise guarantee covers the use of ab15603 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

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    WB

     WB: 1/20 - 1/50.Predicte...Read more →

    WB: 1/20 - 1/50.Predicted molecular weight: 185.4 kDa.

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    Flow Cyt

     Flow Cyt: Use at an assa...Read more →

    Flow Cyt: Use at an assay dependent dilution.

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    ICC

     ICC: 1/20 - 1/50.(Aceton...Read more →

    ICC: 1/20 - 1/50.(Acetone fixed cytospin preparations.)

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    IHC-P

     IHC-P: 1/20Perform heat ...Read more →

    IHC-P: 1/20Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

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    IHC-Fr

     IHC-Fr: 1/20(Acetone fix...Read more →

    IHC-Fr: 1/20(Acetone fixed.)

Target

Function

Mediates hepatobiliary excretion of numerous organic anions. May function as a cellular cisplatin transporter.

Tissue specificity

Found on the apical membrane of polarized cells in liver, kidney and intestine. The highest expression is found in liver.

Involvement in disease

Defects in ABCC2 are the cause of Dubin-Johnson syndrome (DJS) [MIM:237500]. DJS is an autosomal recessive disorder characterized by conjugated hyperbilirubinemia, an increase in the urinary excretion of coproporphyrin isomer I, deposition of melanin-like pigment in hepatocytes, and prolonged retention of sulfobromophthalein, but otherwise normal liver function.

Sequence similarities

Belongs to the ABC transporter superfamily. ABCC family. Conjugate transporter (TC 3.A.1.208) subfamily.
Contains 2 ABC transmembrane type-1 domains.
Contains 2 ABC transporter domains.

Cellular localization

Membrane.

Target information above from: UniProt accessionQ92887 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • ABC30 antibody
  • ABCC2 antibody
  • ATP binding cassette sub family C (CFTR/MRP) member 2 antibody
  • ATP binding cassette subfamily C member 2 antibody
  • ATP-binding cassette sub-family C member 2 antibody
  • Canalicular multidrug resistance protein antibody
  • Canalicular multispecific organic anion transporter 1 antibody
  • CMOAT antibody
  • CMOAT1 antibody
  • cMRP antibody
  • DJS antibody
  • KIAA1010 antibody
  • MRP 2 antibody
  • MRP2_HUMAN antibody
  • Multidrug resistance associated protein 2 antibody
  • Multidrug resistance-associated protein 2 antibody
see all

Database links

References for Anti-MRP2 antibody [M2III-5] (ab15603)

This product has been referenced in:

  • Saeki Jet al. LPS-induced dissociation of multidrug resistance-associated protein 2 (Mrp2) and radixin is associated with Mrp2 selective internalization in rats. Biochem Pharmacol 81:178-84 (2011). WB, IHC-Fr; Rat.Read more (PubMed: 20868650) »
  • Hardwick RNet al. Variations in ABC-Transporter Regulation during the Progression of Human Nonalcoholic Fatty Liver Disease. Drug Metab Dispos : (2011). WB; Human.Read more (PubMed: 21878559) »

See all 5 publications for this product

Publishing research using ab15603? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"