Anti-MRP4 antibody [M4I-10] (ab15602)

Do you have images of Western blots using these two MRP4 antibodies, ab15602 and ab15598?

ANSWER:

Thank you for your enquiry.

The publication found at the following link contains images of Western blots for both of these antibodies:

http://mcb.asm.org/cgi/content/full/24/17/7612?view=long&pmid=15314169

I hope this helps. Please contact us if you have any more questions.

Please recommend a positive control for WB in HEK (human embryonic kidney) cells. I will use AB ab15602 or ab15598. Thanks

Could you also please advise me on a positive control for MRP5 (WB, human cells). Thanks. MRP5; ab3377

ANSWER:

Thank you for your enquiry.

I am sorry for the delay to your enquiry; I have been in touch with the source of this antibody and a response has not been forthcoming. For the testing of antibodies ab15598 and ab15602 against MRP4 I have been informed that HEK-cells transfected with MRP4 can be used. Furthermore MRP4 can be detected in HEK-cells alone. They have also provided me with the recommendation of kidney tissue.

With regards ab3377 and a positive control lysate I am sorry but I have not been able to source information such that I am enable to suggest a positive control lysate. I apologise for this. I would like to suggest that you perform a search of the literature.

I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

We have a customer calling about one of your products ab15602. Customer was doing testing on Western Blot.

The test methods he used are as follows:

100 µg of b16 cancer cell lysate Customer used both 1% milk and also used 1% BSA Primary Ab dilution 1:100

No band was detected. The lot number is 120445. Do you have any suggestions or if possible, send us another vial of different lot for replacement?

ANSWER:

Thank you for contacting us for technical support with ab15602.

I am currently trying to find out the recommended positive control for this antibody and as soon as I have this information I will let you know. The antibody is supplied as tissue culture supernatant therefore the recommended dilutions are in the range of 1:20-50 (overnight incubation), please make sure you dilute in TBS Tween20 0.1% and incubate at 4C.

The problem may also be due to poor extraction of the protein from the cell membrane, please make sure you use a strong detergent (e.g NP40) in the lysis buffer containing protease inhibitors. Without further protocol details it is very hard to know what the problem might be and if the customer still experiences problem please guide him to the link below where a questionnaire will help him to put all the details easily together and we can investigate this matter in details,

http://www.abcam.com/index.html?section=western&pageconfig=technical&intAbID=15602&mode=questionaire

Please do not hesitate to contact me if you require further help,

I have questions for Ab 15602 Please could you give me informations : have you heard about cross reactivity with the rat MARP4 with this Ab ? Is there any unspecificity problems on detecting this protein on tha rat ? as seen on a recent paper : molecular and cellular biology, sept. 2004 p 7612-7621

Thanks

ANSWER:

Thank you for your enquiry regarding ab15602. This antibody has not been tested in rat (only human and mouse) but we would not advise to use it in rat in IHC as the antibody has been raised in rat and hence the secondary antibody anti-rat is very likely to bind to all the rat tissue and give non specific binding.

I hope this information helps, please do not hesitate to contact us if you need any more advice or information,