Feedback Form

Product Name 

MRP4 antibody [M4I-10]

 

See all MRP4 antibodies (6)...

Product type 

Primary antibodies

Description 

Rat monoclonal [M4I-10] to MRP4

Immunogen 

This antibody was selected after immunization with a fusion protein containing the E. coli maltose binding protein and a fragment of the human MRP4 protein corresponding to amino acids 372-431.

Reacts with 
(species key)

Hu, Ms

Tested applications 
(see key)

ICC, IHC-Fr, WB


Abreviews 

Customer reviews
(see key)


Read about Abreviews

Submit an Abreview

Be the first to review this antibody and earn 220 Abpoints.


Application notes 
(see key)

Recommended dilutions
ICC: 1/20 - 1/50 (acetone fixed cytospin preparations).
IHC-Fr :1/20. Fix with acetone, incubate with biotin-labeled rabbit anti-rat IgG and HRP-labeled streptavidin.
WB: 1/20 - 1/50. Predicted molecular weight: 159 kDa.

Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Kidney tissue

Cellular localization 

Cell Membrane

Research areas 

Cancer >> Drug resistance >> MRP-related proteins
Signal Transduction >> Metabolism >> Drug metabolism
Signal Transduction >> Metabolism >> Plasma Membrane >> Channels
Signal Transduction >> Metabolism >> Plasma Membrane >> ATPases

Relevance 

ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). MRP4 is a member of the MRP subfamily which is involved in multi-drug resistance. MRP4 may play a role in cellular detoxification as a pump for its substrate, organic anions. Alternative splicing results in multiple splice variants encoding different isoforms.

 

Alternative names MRP4 antibody [M4I-10] (ab15602)

Database links 

The links below go to external sites and will open in a new browser window

Entrez Gene

    

10257    (Human)
239273    (Mouse)

GeneCard

    

GC13M094470     (Human)

Omim

    

605250    (Human)

SwissProt

    

O15439    (Human)

Raised in 

Rat

Clonality 

Monoclonal

Clone number 

M4I-10

Isotype 

IgG2a

Purity 

Tissue culture supernatant

Storage buffer 

Preservative: 0.1% Sodium Azide
Constituents: 0.7% BSA, Tissue culture supernatant

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

0.150 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

 

At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this MRP4 antibody [M4I-10] is on this datasheet. But please do contact us if you would like any reassurance!



References for MRP4 antibody [M4I-10] (ab15602)

Specific publication references   This product has been used in: (two most recent references)

Ose A et al. Limited brain distribution of [3R,4R,5S]-4-acetamido-5-amino-3-(1-ethylpropoxy)-1-cyclohexene-1-carboxylate phosphate (Ro 64-0802), a pharmacologically active form of oseltamivir, by active efflux across the blood-brain barrier mediated by organic anion transporter 3 (Oat3/Slc22a8) and multidrug resistance-associated protein 4 (Mrp4/Abcc4). Drug Metab Dispos 37:315-21 (2009). PubMed: 19029202

Lim JC et al. Decreased expression of multidrug efflux transporters in the brains of GSK-3beta transgenic mice. Brain Res 1276:1-10 (2009). IHC-Fr; Mouse. PubMed: 19393634


See all 7 publication references for this product.


If you publish research using ab15602 please let us know so that we can cite the reference on this datasheet.

 

Search PubMed (MEDLINE) for references to MRP4

 

MRP4 antibody [M4I-10] - more information

Customer reviews (feedback) regarding MRP4 antibody [M4I-10]

Customer FAQs regarding MRP4 antibody [M4I-10]

Protocols for MRP4 antibody [M4I-10]

Price and availability of products related to MRP4 antibody [M4I-10]

 

MRP4 antibody [M4I-10] - related products:

Products for Immunocytochemistry

Subcellular Markers

Products for Western blot

Loading controls

Lysates

 

Compatible Secondaries for MRP4 antibody [M4I-10]

ab6565

  

Cy5 ® antibody

ab6730

  

FITC antibody

ab6733

  

Biotin antibody

ab6734

  

HRP antibody

ab6843

  

Texas Red ® antibody

ab6846

  

Alkaline Phosphatase antibody

ab7010

  

Phycoerythrin antibody

ab7020

  

Agarose antibody

 

Other MRP4 Antibodies

See all MRP4 antibodies (6)...

 

MRP4 antibody [M4I-10] - other tools:

Contact Abcam with a Technical Enquiry about MRP4 antibody [M4I-10]

 

All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

©1998-2010 Abcam plc. All rights reserved.