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Product Name
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MRP4 antibody [M4I-10]
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See all MRP4 antibodies (6)...
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Product type
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Primary antibodies
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Description
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Rat monoclonal [M4I-10] to MRP4
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Immunogen
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This antibody was selected after immunization with a fusion protein containing the E. coli maltose binding protein and a fragment of the human MRP4 protein corresponding to amino acids 372-431.
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Reacts with
(species key)
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Hu, Ms
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Tested applications
(see key)
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ICC, IHC-Fr, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ICC: 1/20 - 1/50 (acetone fixed cytospin preparations).
IHC-Fr :1/20. Fix with acetone, incubate with biotin-labeled rabbit anti-rat IgG and HRP-labeled streptavidin.
WB: 1/20 - 1/50. Predicted molecular weight: 159 kDa.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Kidney tissue
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Cellular localization
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Cell Membrane
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Research areas
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Cancer >> Drug resistance >> MRP-related proteins
Signal Transduction >> Metabolism >> Drug metabolism
Signal Transduction >> Metabolism >> Plasma Membrane >> Channels
Signal Transduction >> Metabolism >> Plasma Membrane >> ATPases
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Relevance
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ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). MRP4 is a member of the MRP subfamily which is involved in multi-drug resistance. MRP4 may play a role in cellular detoxification as a pump for its substrate, organic anions. Alternative splicing results in multiple splice variants encoding different isoforms.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rat
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Clonality
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Monoclonal
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Clone number
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M4I-10
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Isotype
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IgG2a
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Purity
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Tissue culture supernatant
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Storage buffer
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Preservative: 0.1% Sodium Azide
Constituents: 0.7% BSA, Tissue culture supernatant Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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0.150 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this MRP4 antibody [M4I-10] is on this datasheet. But please do contact us if you would like any reassurance! |
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References for MRP4 antibody [M4I-10] (ab15602)
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This product has been used in: (two most recent references)
Ose A et al. Limited brain distribution of [3R,4R,5S]-4-acetamido-5-amino-3-(1-ethylpropoxy)-1-cyclohexene-1-carboxylate phosphate (Ro 64-0802), a pharmacologically active form of oseltamivir, by active efflux across the blood-brain barrier mediated by organic anion transporter 3 (Oat3/Slc22a8) and multidrug resistance-associated protein 4 (Mrp4/Abcc4). Drug Metab Dispos 37:315-21 (2009). PubMed: 19029202
Lim JC et al. Decreased expression of multidrug efflux transporters in the brains of GSK-3beta transgenic mice. Brain Res 1276:1-10 (2009). IHC-Fr; Mouse. PubMed: 19393634
See all 7 publication references for this product.
If you publish research using ab15602 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to MRP4
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