Anti-MRPL1 antibody (ab232886)
Key features and details
- Rabbit polyclonal to MRPL1
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-MRPL1 antibody
See all MRPL1 primary antibodies -
Description
Rabbit polyclonal to MRPL1 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment (His-T7-tag) corresponding to Human MRPL1 aa 100-300. N-terminal tags. Expressed in E. coli.
Database link: Q9BYD6 -
Positive control
- ICC/IF: HepG2 cells. IHC-P: Human lung cancer tissue. WB: Recombinant human MRPL1 protein. MCF7 and HepG2 cell lysate.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.011% Proclin 300
Constituents: 55.77% Glycerol (glycerin, glycerine), 44.219% PBS -
Concentration information loading...
-
Purity
Protein A purified -
Purification notes
Antigen-specific affinity chromatography followed by Protein A affinity chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab232886 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
ICC/IF |
Use a concentration of 5 - 20 µg/ml.
|
|
IHC-P |
Use a concentration of 5 - 20 µg/ml.
|
|
WB |
Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 37 kDa.
|
Notes |
---|
ICC/IF
Use a concentration of 5 - 20 µg/ml. |
IHC-P
Use a concentration of 5 - 20 µg/ml. |
WB
Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 37 kDa. |
Target
-
Sequence similarities
Belongs to the ribosomal protein L1P family. -
Cellular localization
Mitochondrion. - Information by UniProt
-
Database links
- Entrez Gene: 65008 Human
- Omim: 611821 Human
- SwissProt: Q9BYD6 Human
- Unigene: 532019 Human
-
Alternative names
- 39S ribosomal protein L1 antibody
- 39S ribosomal protein L1, mitochondrial precursor antibody
- BM022 antibody
see all
Images
-
All lanes : Anti-MRPL1 antibody (ab232886) at 1 µg/ml
Lane 1 : MCF7 (human breast adenocarcinoma cell line) cell lysate
Lane 2 : HepG2 (human liver hepatocellular carcinoma cell line) cell lysate
Secondary
All lanes : HRP-linked Guinea pig anti-Rabbit at 1/2000 dilution
Predicted band size: 37 kDa -
Formalin-fixed, paraffin-embedded human lung cancer tissue stained for MRPL1 with ab232886 at 20 µg/ml in immunohistochemical analysis. DAB staining.
-
HepG2 (Human liver hepatocellular carcinoma cell line) cells stained for MRPL1 using ab232886 at 20 µg/ml in ICC/IF. FITC stained.
-
Anti-MRPL1 antibody (ab232886) at 2 µg/ml + Recombinant human MRPL1 protein
Predicted band size: 37 kDa
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
SDS download
-
Datasheet download
References (0)
ab232886 has not yet been referenced specifically in any publications.