Recombinant Anti-MRPL20 antibody [EPR12592] (ab181058)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12592] to MRPL20
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
-
Product name
Anti-MRPL20 antibody [EPR12592]
See all MRPL20 primary antibodies -
Description
Rabbit monoclonal [EPR12592] to MRPL20 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- HUVEC, HeLa, C6, RAW 264.7, PC12 and NIH 3T3 cell lysates.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA -
Concentration information loading...
-
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR12592 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Isotype control
-
Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab181058 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/1000 - 1/2000. Detects a band of approximately 17 kDa (predicted molecular weight: 17 kDa).
|
Notes |
---|
WB
1/1000 - 1/2000. Detects a band of approximately 17 kDa (predicted molecular weight: 17 kDa). |
Target
-
Relevance
MRPL20 is one of more than 70 protein components of mitochondrial ribosomes that are encoded by the nuclear genome. MRPL20 is a subunit of the 39S mitochondrial ribosome. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. -
Cellular localization
Mitochondrial -
Database links
- Entrez Gene: 55052 Human
- Entrez Gene: 66448 Mouse
- Entrez Gene: 680747 Rat
- Omim: 611833 Human
- SwissProt: Q9BYC9 Human
- SwissProt: Q9CQL4 Mouse
-
Alternative names
- 39S ribosomal protein L20, mitochondrial antibody
- L20mt antibody
- MGC4779 antibody
see all
Images
-
All lanes : Anti-MRPL20 antibody [EPR12592] (ab181058) at 1/1000 dilution
Lane 1 : HUVEC cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : C6 cell lysate
Lane 4 : RAW 264.7 cell lysate
Lane 5 : PC12 cell lysate
Lane 6 : NIH 3T3 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L) HRP at 1/1000 dilution
Predicted band size: 17 kDa
Datasheets and documents
-
SDS download
-
Datasheet download
Certificate of Compliance
References (0)
ab181058 has not yet been referenced specifically in any publications.