Overview
- Product nameAnti-MRPS2 antibody [4D6]See all MRPS2 primary antibodies ...
- DescriptionMouse monoclonal [4D6] to MRPS2
- Tested applicationsWB, IHC-P, Flow Cyt more details
- Species reactivityReacts with: Human
- Immunogen
Full length Human recombinant MRPS2 (NP_057118) produced in E.coli.
- Positive controlHuman lung carcinoma tissue. Lysate from transfected HEK293T cells. HeLa, Jurkat or transfected HEK293T cells.
Properties
- FormLiquid
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
- Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 1% BSA, 48% PBS, 50% Glycerol -
Concentration information loading... - PurityImmunogen affinity purified
- Purification notesPurified from mouse ascites fluids by affinity chromatography
- Clonality Monoclonal
- Clone number4D6
- IsotypeIgG2a
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab117897 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| WB | WB: 1/2000. Predicted molecular weight: 33 kDa. |
| IHC-P | IHC-P: 1/150. |
| Flow Cyt | Flow Cyt: 1/100. |
Target
- Sequence similaritiesBelongs to the ribosomal protein S2P family.
- Cellular localizationMitochondrion.
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Database links
- Entrez Gene: 51116 Human
- Omim: 611971 Human
- SwissProt: Q9Y399 Human
- Unigene: 382044 Human
Target information above from: UniProt accession
Q9Y399
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
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Alternative names
- 28S ribosomal protein S2 antibody28S ribosomal protein S2 mitochondrial antibodyCGI 91 antibody
- CGI91 antibodyMitochondrial 28S ribosomal protein S2 antibodymitochondrial antibodyMitochondrial ribosomal protein S2 antibodyMRP S2 antibodyMRP-S2 antibodyMRPS2 antibodyRT02_HUMAN antibodyS2mt antibody
see all
Anti-MRPS2 antibody [4D6] images
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All lanes : Anti-MRPS2 antibody [4D6] (ab117897) at 1/2000 dilution
Lane 1 : mock-transfected HEK293T cell lysate
Lane 2 : MRPS2-transfected HEK293T cell lysate
Lysates/proteins at 5 µg per lane.
Predicted band size : 33 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRPS2 antibody [4D6] (ab117897)Immunohistochemical staining of paraffin-embedded Human lung carcinoma tissue using 1/150 ab117897. -
HEK293T cells transfected with either MRPS2-expressing vector (Red) or empty vector control plasmid (Blue) were immunostained with 1/100 ab117897, and then analyzed by flow cytometry.
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Flow cytometric analysis of Hela cells, using 1/100 ab117897 (Red), compared to a nonspecific negative control antibody (Blue).
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Flow cytometric analysis of Jurkat cells, using 1/100 ab117897 (Red), compared to a nonspecific negative control antibody (Blue).
References for Anti-MRPS2 antibody [4D6] (ab117897)
ab117897 has not yet been referenced specifically in any publications.
![Western blot - Anti-MRPS2 antibody [4D6] (ab117897) Western blot - Anti-MRPS2 antibody [4D6] (ab117897)](http://a.abcam.com/ps/datasheet/images/117/ab117897/MRPS2-Primary-antibodies-ab117897-1.jpg)
![Flow Cytometry - Anti-MRPS2 antibody [4D6] (ab117897) Flow Cytometry - Anti-MRPS2 antibody [4D6] (ab117897)](http://a.abcam.com/ps/datasheet/images/117/ab117897/MRPS2-Primary-antibodies-ab117897-3.jpg)
![Flow Cytometry - Anti-MRPS2 antibody [4D6] (ab117897) Flow Cytometry - Anti-MRPS2 antibody [4D6] (ab117897)](http://a.abcam.com/ps/datasheet/images/117/ab117897/MRPS2-Primary-antibodies-ab117897-4.jpg)
![Flow Cytometry - Anti-MRPS2 antibody [4D6] (ab117897) Flow Cytometry - Anti-MRPS2 antibody [4D6] (ab117897)](http://a.abcam.com/ps/datasheet/images/117/ab117897/MRPS2-Primary-antibodies-ab117897-5.jpg)