Overview
- Product nameAnti-MRPS25 antibodySee all MRPS25 primary antibodies ...
- DescriptionMouse monoclonal to MRPS25
- Tested applicationsWB, ELISA more details
- Species reactivityReacts with: Mouse
Predicted to work with: Human - Immunogen
Recombinant full length Human MRPS25 protein (AAH03590) with a 26kDa tag.
- Positive controlNIH/3T3 cell lysate. Recombinant tagged Human MRPS25 protein.
Properties
- FormLiquid
- Storage instructionsStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
- Storage bufferPreservative: None
Constituents: 1X PBS, pH 7.2 -
Concentration information loading... - PurityProtein A purified
- Clonality Monoclonal
- IsotypeIgG2a
- Light chain typekappa
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab77447 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| WB | WB: Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 20 kDa (predicted molecular weight: 20 kDa). |
| ELISA | ELISA: Use at an assay dependent dilution. |
Target
- Sequence similaritiesBelongs to the ribosomal protein S25/L51 family.
- Cellular localizationMitochondrion.
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Database links
- Entrez Gene: 64432 Human
- Entrez Gene: 64658 Mouse
- Omim: 611987 Human
- SwissProt: P82663 Human
- SwissProt: Q9D125 Mouse
- Unigene: 715617 Human
- Unigene: 476867 Mouse
- Unigene: 87062 Mouse
Target information above from: UniProt accession
P82663
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
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Alternative names
- 28S ribosomal protein S25 antibody28S ribosomal protein S25 mitochondrial antibodyMitochondrial 28S ribosomal protein S25 antibody
- mitochondrial antibodyMitochondrial ribosomal protein S25 antibodyMRP S25 antibodyMRP-S25 antibodyMRPS25 antibodyRPMS25 antibodyRT25_HUMAN antibodyS25mt antibody
see all
Anti-MRPS25 antibody images
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Western blot - MRPS25 antibody (ab77447)Anti-MRPS25 antibody (ab77447) at 1 µg/ml + NIH/3T3 cell lysate at 25 µg
Secondary
Goat anti-mouse IgG (H&L)-HRP conjugate at 1/2500 dilution
Predicted band size : 20 kDa
Observed band size : 20 kDa -
Western blot - MRPS25 antibody (ab77447)Anti-MRPS25 antibody (ab77447) at 1 µg/ml + Recombinant tagged Human MRPS25 protein at 0.2 µg
Secondary
Goat anti-mouse IgG (H&L)-HRP conjugate at 1/5000 dilution
Predicted band size : 20 kDa
Observed band size : 42 kDa (why is the actual band size different from the predicted?)
References for Anti-MRPS25 antibody (ab77447)
ab77447 has not yet been referenced specifically in any publications.
