Overview

  • Product nameAnti-MRPS25 antibodySee all MRPS25 primary antibodies ...
  • Description
    Mouse monoclonal to MRPS25
  • Tested applicationsWB, ELISAmore details
  • Species reactivity
    Reacts with: Mouse
    Predicted to work with: Human
  • Immunogen

    Recombinant full length Human MRPS25 protein (AAH03590) with a 26kDa tag.

  • Positive control
    • NIH/3T3 cell lysate. Recombinant tagged Human MRPS25 protein.

Properties

Applications

Our Abpromise guarantee covers the use of ab77447 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 20 kDa (predicted molecular weight: 20 kDa).
ELISA Use at an assay dependent dilution.

Target

Anti-MRPS25 antibody images

  • Anti-MRPS25 antibody (ab77447) at 1 µg/ml + NIH/3T3 cell lysate at 25 µg

    Secondary
    Goat anti-mouse IgG (H&L)-HRP conjugate at 1/2500 dilution

    Predicted band size : 20 kDa
    Observed band size : 20 kDa
  • Anti-MRPS25 antibody (ab77447) at 1 µg/ml + Recombinant tagged Human MRPS25 protein at 0.2 µg

    Secondary
    Goat anti-mouse IgG (H&L)-HRP conjugate at 1/5000 dilution

    Predicted band size : 20 kDa
    Observed band size : 42 kDa (why is the actual band size different from the predicted?)

References for Anti-MRPS25 antibody (ab77447)

ab77447 has not yet been referenced specifically in any publications.

Product Wall

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"