Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> Mismatch Repair
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ab52266 has been referenced in 2 publications.
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Immunohistochemical analysis of paraffin-embedded human rectum carcinoma tissue, showing nuclear and cytoplasmic localisation, using ab52266 at a dilution of 1/200 - 1/1000 with DAB staining.
ab52266 (1/200) detecting MSH2 in HeLa cells (green). Cells were fixed in methanol (-20'C, 10min) and counterstained with DAPI in order to highlight the nucleus. Please refer to abreview for further experimental details.
This image is courtesy of an Abreview submitted by Dr Kirk McManus
All lanes : Anti-MSH2 antibody [3A2B8C] (ab52266) at 1/2000 dilution
Lane 1 : Cell lysates prepared from human Hela cells
Lane 2 : Cell lysates prepared from A549 cells
Lane 3 : Cell lysates prepared from human A431 cells
Lane 4 : Cell lysates prepared from HEK293 cells
Lysates/proteins at 100 µg per lane.
Secondary
HRP-conjugated Goat polyclonal to mouse IgG
Predicted band size : 105 kDa
ab52266 at 1/1000 dilution staining MSH2 in human Hela cells by Immunocytochemistry/ Immunofluorescence. An Alexa Fluor® 488 conjugated Goat polyclonal to mouse IgG1 was used as secondary antibody. The primary antibody shows green staining in image whilst actin filaments were stained red with Alexa Fluor® 555 phalloidin.
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