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Immunohistochemistry (Paraffin-embedded sections) - MSH2 antibody [3A2B8C] (ab52266)
![Immunohistochemistry (Paraffin-embedded sections) - MSH2 antibody [3A2B8C] (ab52266)](/ps/datasheet/images/ab52266ihc.jpg)
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Western blot - MSH2 antibody [3A2B8C] (ab52266)
![Western blot - MSH2 antibody [3A2B8C] (ab52266)](/ps/datasheet/images/ab52266wb.jpg)
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Immunocytochemistry/ Immunofluorescence - MSH2 antibody [3A2B8C] (ab52266)
![Immunocytochemistry/ Immunofluorescence - MSH2 antibody [3A2B8C] (ab52266)](/ps/datasheet/images/ab52266_rev9156_2.jpg)
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Product Name
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MSH2 antibody [3A2B8C]
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See all MSH2 antibodies (9)...
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Product type
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Primary antibodies
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Description
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Mouse monoclonal [3A2B8C] to MSH2
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Immunogen
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Ni-NTA purified recombinant human MSH2 expressed in E. Coli strain BL21 (DE3).
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Reacts with
(species key)
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Hu
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Tested applications
(see key)
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ICC/IF, IHC-Fr, IHC-P, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ICC/IF: Use at an assay dependent dilution.
IHC-Fr: 1/200 - 1/1000.
IHC-P: 1/200 - 1/1000.
WB: 1/200 - 1/1000. Detects a band of approximately 98 kDa (predicted molecular weight: 105 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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A431 cell lysate; human rectum carcinoma tissue.
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Cellular localization
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Nuclear
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Research areas
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Cancer >> Oncoproteins/suppressors >> Tumor suppressors >> Other
Chromatin and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> Mismatch Repair
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Relevance
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MSH2 is involved in DNA repair as a mismatch repair protein, and mutations of MSH2 are found in approximately 50% of inherited non polyposis colorectal carcinoma (HNPCC) (Lynch syndrome) cases. HNPCC is an autosomal, dominantly inherited disease associated with marked increase in cancer susceptibility. It is characterized by a familial predisposition to early onset colorectal carcinoma and extra-colonic cancers of the gastrointestinal, urological and female reproductive tracts. HNPCC is reported to be the most common form of inherited colorectal cancer in the western world.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Mouse
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Clonality
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Monoclonal
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Clone number
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3A2B8C
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Isotype
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IgG1
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Purity
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Protein G purified
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this MSH2 antibody [3A2B8C] is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for MSH2 antibody [3A2B8C] images, references, products related to ab52266 and other tools.
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MSH2 antibody [3A2B8C] images:
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 Immunohistochemistry (Paraffin-embedded sections) - MSH2 antibody [3A2B8C] (ab52266)
Immunohistochemical analysis of paraffin-embedded human rectum carcinoma tissue, showing nuclear and cytoplasmic localisation, using ab52266 at a dilution of 1/200 - 1/1000 with DAB staining.
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Western blot - MSH2 antibody [3A2B8C] (ab52266)
Lane 1 : Marker
Lane 2 : MSH2 antibody [3A2B8C] (ab52266) at 1/200 dilution
Lane 1 : As above
Lane 2 : A431 cells
Predicted band size : 105 kDa
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Immunocytochemistry/ Immunofluorescence - MSH2 antibody [3A2B8C] (ab52266)
ab52266 (1/200) detecting MSH2 in HeLa cells (green). Cells were fixed in methanol (-20'C, 10min) and counterstained with DAPI in order to highlight the nucleus. Please refer to abreview for further experimental details. This image is courtesy of an Abreview submitted by Dr Kirk McManus
See Abreview
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References for MSH2 antibody [3A2B8C] (ab52266)
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This product has been used in:
Svendsen JM et al. Mammalian BTBD12/SLX4 assembles a Holliday junction resolvase and is required for DNA repair. Cell 138:63-77 (2009). WB; Human. PubMed: 19596235
If you publish research using ab52266 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to MSH2
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MSH2 antibody [3A2B8C] - more information
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Customer reviews (feedback) regarding MSH2 antibody [3A2B8C] |
Customer FAQs regarding MSH2 antibody [3A2B8C] |
Protocols for MSH2 antibody [3A2B8C] |
Price and availability of products related to MSH2 antibody [3A2B8C] |
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MSH2 antibody [3A2B8C] - related products:
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MSH2 antibody [3A2B8C] - other tools:
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Contact Abcam with a Technical Enquiry about MSH2 antibody [3A2B8C] |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Pricing information loading
![Immunohistochemistry (Paraffin-embedded sections) - MSH2 antibody [3A2B8C] (ab52266)](/index.html?datasheet=52266#ab52266ihc.jpg)
![Western blot - MSH2 antibody [3A2B8C] (ab52266)](/index.html?datasheet=52266#ab52266wb.jpg)
![Immunocytochemistry/ Immunofluorescence - MSH2 antibody [3A2B8C] (ab52266)](/index.html?datasheet=52266#ab52266_rev9156_2.jpg)
