If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Publishing research using ab16833? Please let us know so that we can cite the reference in this datasheet
ab16833 has been referenced in 2 publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Anti-MSH2 antibody (ab16833) at 1/500 dilution + Human Glioblastoma whole cell lysate
Secondary
HRP-conjugated Goat anti-Rabbit at 1/7500 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 100 kDa
Exposure time : 30 minutes
This image is courtesy of an anonymous Abreview
IHC image of ab16833 staining in human seminoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab16833, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
1
Call 01223 696 000 or contact us