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I am curious as to which part of the MSH2 protein the antibody is raised against. I am interested in using this antibody to probe for Plasmodium falciparum MSH2, and would like to know whether it is against a conserved sequence. |
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ANSWER: |
Thank you for your enquiry. This antibody was raised against full length recombinant MSH2 protein. Since it is a polyclonal antibody, it will consist of a mixed population which will recognise different epitopes across the entire protein. I hope this information helps, please do not hesitate to contact us if you need any more advice or information. |
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Anti-MSH2 antibody (ab16833) at 1/500 dilution + Human Glioblastoma whole cell lysate
Secondary
HRP-conjugated Goat anti-Rabbit at 1/7500 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 100 kDa
Exposure time : 30 minutes
This image is courtesy of an anonymous Abreview
IHC image of ab16833 staining in human seminoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab16833, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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