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Anti-MSK1 (phospho S376) antibody [E375] (ab32190)

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Overview

Product name

Anti-MSK1 (phospho S376) antibody [E375]
See all MSK1 products (10) ...

Description

Rabbit monoclonal [E375] to MSK1 (phospho S376)

Specificity

This antibody will detect MSK1 phosphorylation on Serine 376.

Tested applications

WB, ICC, IP, IHC-Pmore details

Cross reactivity

Reacts with

Human

Does not react with

Mouse, Rat

Immunogen

A synthetic phospho peptide corresponding to residues surrounding Serine 376 of human MSK1.

Positive control

WB: K562 cell lysate. IHC-P Human urinary bladder carcinoma.

General notes

Produced under U.S. Patent No. 5,675,063.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Storage buffer

PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%

Concentration

Concentration information loading...

Clonality

Monoclonal

Clone number

E375

Isotype

IgG

Research areas

Metabolism >> Pathways and Processes >> Redox metabolism >> Oxidative stress
Cell Biology >> Other Antibodies >> Oxidative Stress
Epigenetics and Nuclear Signaling >> Transcription >> Other factors
Signal Transduction >> Protein Phosphorylation >> Tyrosine Kinases >> Receptor Tyrosine Kinases
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> Other Kinases

  • Western blot - MSK1 (phospho S376) antibody [E375] (ab32190)Western blot - MSK1 (phospho S376) antibody [E375] (ab32190) image (enlarge)

  • Immunohistochemistry (Paraffin-embedded sections) - MSK1 (phospho S376) antibody [E375] (ab32190)Immunohistochemistry (Paraffin-embedded sections) - MSK1 (phospho S376) antibody [E375] (ab32190) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab32190 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application notes

ICC: 1/100 - 1/250.
IHC-P: 1/50 - 1/100.
IP: 1/30.
WB: 1/1000 - 1/5000. Detects a band of approximately 87 kDa (predicted molecular weight: 90 kDa).

Is unsuitable for FACS.

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Target

Function

Serine/threonine kinase required for the mitogen or stress-induced phosphorylation of the transcription factors CREB (cAMP response element-binding protein) and ATF1 (activating transcription factor-1). Essential role in the control of RELA transcriptional activity in response to TNF. Directly represses transcription via phosphorylation of 'Ser-1' of histone H2A. Phosphorylates 'Ser-10' of histone H3 in response to mitogenics, stress stimuli and epidemal growth-factor (EGF), which results in the transcriptional activation of several immediate early genes, including proto-oncogenes c-fos/FOS and c-jun/JUN. May also phosphorylate 'Ser-28' of histone H3. Mediates the mitogen- and stress-induced phosphorylation of high mobility group protein 14 (HMG-14).

Tissue specificity

Widely expressed with high levels in heart, brain and placenta. Less abundant in lung, kidney and liver.

Sequence similarities

Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 2 protein kinase domains.

Post-translational
modifications

Ser-376 and Thr-581 phosphorylation is required for kinase activity. Ser-376 and Ser-212 are autophosphorylated by the C-terminal kinase domain, and their phosphorylation is essential for the catalytic activity of the N-terminal kinase domain.

Cellular localization

Nucleus. Cytoplasm. Predominantly nuclear. Partially cytoplasmic.

Target information above from: UniProt accessionO75582 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • S6K alpha 5 antibody
  • 90 kDa Ribosomal Protein S6 Kinase 5 antibody
  • EC 2.7.11.1 antibody
  • KS6A5_HUMAN antibody
  • MGC1911 antibody
  • Mitogen and stress activated protein kinase 1 antibody
  • MSPK1 antibody
  • Nuclear Mitogen And Stress Activated Protein Kinase 1 antibody
  • Nuclear mitogen- and stress-activated protein kinase 1 antibody
  • Ribosomal protein S6 kinase 90kD polypeptide 5 antibody
  • Ribosomal protein S6 kinase 90kDa antibody
  • Ribosomal protein S6 kinase 90kDa polypeptide 5 antibody
  • Ribosomal Protein S6 Kinase Alpha 5 antibody
  • Ribosomal protein S6 kinase alpha-5 antibody
  • RLPK antibody
  • RPS6KA5 antibody
  • RSK Like Protein Kinase antibody
  • RSK-like protein kinase antibody
  • RSKL antibody
  • S6K-alpha-5 antibody
see all

Database links

Anti-MSK1 (phospho S376) antibody [E375] images:

  Western blot - MSK1 (phospho S376) antibody [E375] (ab32190)

Western blot - MSK1 (phospho S376) antibody [E375] (ab32190)

All lanes : Anti-MSK1 (phospho S376) antibody [E375] (ab32190) at 1/5000 dilution

Lane 1 : Untreated K562 cell lysate
Lane 2 : K562 cell lysate treated with Alkaline


Predicted band size : 90 kDa
Observed band size : 87 kDa (why is the actual band size different from the predicted?)

  Immunohistochemistry (Paraffin-embedded sections) - MSK1 (phospho S376) antibody [E375] (ab32190)

Immunohistochemistry (Paraffin-embedded sections) - MSK1 (phospho S376) antibody [E375] (ab32190)

Ab32190, at a dilution of 1/50, staining MSK1 in paraffin embedded human urinary bladder carcinoma tissue by Immunohistochemistry.

References for Anti-MSK1 (phospho S376) antibody [E375] (ab32190)

ab32190 has not yet been referenced specifically in any publications.

Publishing research using ab32190? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"