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Western blot - MT-ND1 antibody (ab74257)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - MT-ND1 antibody (ab74257)
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Product Name
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MT-ND1 antibody
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to MT-ND1
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Immunogen
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A synthesized peptide derived from an internal region of human MT-ND1.
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Reacts with
(species key)
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Hu
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Specificity
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This antibody detects endogenous levels of total MT-ND1 protein.
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Tested applications
(see key)
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ELISA, IHC-P, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: 1/40000.
IHC-P: 1/50 - 1/100.
WB: 1/500 - 1/1000. Predicted molecular weight: 36 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Human placenta tissue and extracts from Jurkat cells.
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Cellular localization
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Mitochondrion inner membrane; Multi pass membrane protein.
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Research areas
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Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Integration of energy metabolism
Signal Transduction >> Metabolism >> Mitochondrial
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Relevance
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MT-ND1 is the core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
Defects in MT-ND1 are a cause of Leber hereditary optic neuropathy (LHON) [MIM:535000]; also known as Leber optic atrophy. LHON is a maternally inherited disease resulting in acute bilateral blindness due to retinal degeneration predominantly in young men. Cardiac conduction defects and neurological defects have also been described, resulting in optic nerve degeneration and cardiac dysrhythmia. Defects in MT-ND1 may also be associated with mitochondrial susceptibility to Alzheimer disease (AD) and non insulin dependent diabetes mellitus (NIDDM).
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MT-ND1 antibody (ab74257) |
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4 Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this MT-ND1 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for MT-ND1 antibody images, references, products related to ab74257 and other tools.
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MT-ND1 antibody images:
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 Western blot - MT-ND1 antibody (ab74257)
All lanes : MT-ND1 antibody (ab74257) at 1/500 dilution
Lane 1 : Extracts from Jurkat cells
Lane 2 : Extracts from Jurkat cells plus 5µg immunizing peptide
Lysates/proteins at 5 µg per lane.
Predicted band size : 36 kDa
Observed band size : 36 kDa
Additional bands at : 21 kDa,55 kDa. We are unsure as to the identity of these extra bands.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - MT-ND1 antibody (ab74257)
Immunohistochemical analysis of MT-ND1 in paraffin embedded human placenta tissue using ab74257 at a 1/50 dilution.
Left image: untreated.
Right image: treated with immunizing peptide.
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Search PubMed (MEDLINE) for references to MT-ND1
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MT-ND1 antibody - more information
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Customer reviews (feedback) regarding MT-ND1 antibody |
Customer FAQs regarding MT-ND1 antibody |
Protocols for MT-ND1 antibody |
Price and availability of products related to MT-ND1 antibody |
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MT-ND1 antibody - related products:
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MT-ND1 antibody - other tools:
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Contact Abcam with a Technical Enquiry about MT-ND1 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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