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Western blot - MT-ND5 antibody (ab83985)
(enlarge)
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Product Name
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MT-ND5 antibody
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to MT-ND5
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Immunogen
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Synthetic peptide corresponding to a region within amino acids 38-87 (SIVASTFIIS LFPTTMFMCL DQEVIISNWH WATTQTTQLS LSFKLDYFSM) of Human MT-ND5 (Swiss-Prot P03915).
BLAST 'SIVASTFIIS LFPTTMFMCL DQEVIISNWH WATTQTTQLS LSFKLDYFSM ' with  or 
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Reacts with
(species key)
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Hu
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Tested applications
(see key)
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WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
WB: Use at a concentration of 1 µg/ml. Predicted molecular weight: 67 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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721_B cell lysate.
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Cellular localization
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Mitochondrion inner membrane; Multi-pass membrane protein.
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Research areas
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Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Integration of energy metabolism
Signal Transduction >> Metabolism >> Mitochondrial
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Relevance
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MT-ND5 is the core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: None
Constituents: 2% Sucrose, PBS
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this MT-ND5 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for MT-ND5 antibody images, references, products related to ab83985 and other tools.
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MT-ND5 antibody images:
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 Western blot - MT-ND5 antibody (ab83985)
MT-ND5 antibody (ab83985) at 1 µg/ml (in 5% skim milk / PBS buffer) + 721_B cell lysate at 10 µg
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 67 kDa
Observed band size : 60 kDa (why is the actual band size different from the predicted?)
Gel concentration: 12%
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Search PubMed (MEDLINE) for references to MT-ND5
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MT-ND5 antibody - more information
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Customer reviews (feedback) regarding MT-ND5 antibody |
Customer FAQs regarding MT-ND5 antibody |
Protocols for MT-ND5 antibody |
Price and availability of products related to MT-ND5 antibody |
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MT-ND5 antibody - related products:
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MT-ND5 antibody - other tools:
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Contact Abcam with a Technical Enquiry about MT-ND5 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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