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Products:Signal Transduction >> Metabolism >> Mitochondrial
Overview
Product name
Anti-MT-ND5 antibody
See all MT-ND5 products (2) ...
Description
Rabbit polyclonal to MT-ND5
Tested applications
ICC/IF, WBmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Chimpanzee
Immunogen
Synthetic peptide corresponding to a region within amino acids 38-87 (SIVASTFIIS LFPTTMFMCL DQEVIISNWH WATTQTTQLS LSFKLDYFSM) of Human MT-ND5 (Swiss-Prot P03915).
SIVASTFIIS LFPTTMFMCL DQEVIISNWH WATTQTTQLS LSFKLDYFSM
Positive control
721_B cell lysate.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 2% Sucrose, PBS
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Integration of energy metabolism
Signal Transduction >> Metabolism >> Mitochondrial
Applications
Show applications key
Our Abpromise guarantee covers the use of ab83985 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use a concentration of 1 µg/ml
WB: Use a concentration of 1 µg/mlPredicted molecular weight: 67 kDa.
Target
Relevance
MT-ND5 is the core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
Cellular localization
Mitochondrion inner membrane; Multi-pass membrane protein
Alternative names
- Complex I, subunit ND5 antibody
- EC 1.6.5.3 antibody
- Mitochondrially encoded NADH dehydrogenase 5 antibody
see all
Database links
- Entrez Gene: 4540 Human
- Omim: 516005 Human
- SwissProt: P03915 Human
Anti-MT-ND5 antibody images:
Western blot - MT-ND5 antibody (ab83985)
Anti-MT-ND5 antibody (ab83985) at 1 µg/ml (in 5% skim milk / PBS buffer) + 721_B cell lysate at 10 µg
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 67 kDa
Observed band size : 60 kDa (why is the actual band size different from the predicted?)
Gel concentration: 12%
Immunocytochemistry/ Immunofluorescence-MT-ND5 antibody(ab83985)
ICC/IF image of ab83985 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab83985, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
References for Anti-MT-ND5 antibody (ab83985)
ab83985 has not yet been referenced specifically in any publications.
Publishing research using ab83985? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"