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Overview

  • Product nameMTA1 protein (Tagged-His Tag)See all MTA1 proteins and peptides ...
  • Protein descriptionRecombinant fragment, corresponding to amino acids 105-452 of Human MTA1 with N terminal His tag; 348 amino acids, 40kDa (39.6kDa predicted from sequence). UniProt ID = Q13330.
  • Expression hostE. coli

    • Properties

  • Purification notesPurity level >85%
    This protein was expressed as an N-terminal His-tag fusion protein using Escherichia coli, and purified using Immobilized Metal Ion Affinity Chromatography. In some cases, smaller protein fragments may be present in addition to the intended expression product as a result of premature termination during translation in E. coli and subsequent co-purification via the His-tag. In some cases purified proteins run at a molecular weight different to the theoretically calculated molecular weight. This may be as a result of unequally distributed charges in the amino acid sequence. Alternatively, dimerisation of the expression product can occur under oxygen limitation during expression/cultivation.
  • FormLyophilised:Reconstitute with 96 µl aqua dest.
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 0.5% Trehalose, 6M Urea, 100mM Sodium phosphate, 10mM Sodium chloride, pH 4.5
  • Concentration information loading...
  • Additional notesProtein Identity confirmed by Mass Spectrometry (MS/MS) (acquired on initial reference batch).
  • Sequence notesHRELFLSRQLESLPATHIRGKCSVTLLNETESLKSYLERE DFFFYSLVYDPQQKTLLADKG EIRVGNRYQADITDLLK EGEEDGRDQSRLETQVWEAHNPLTDKQIDQFLVVARSVGT FARA LDCSSSVRQPSLHMSAAAASRDITLFHAMDTLHK NIYDISKAISALVPQGGPVLCRDEMEE WSASEANLFEE ALEKYGKDFTDIQQDFLPWKSLTSIIEYYYMWKTTDRYVQ QKRLKAAEAE SKLKQVYIPNYNKPNPNQISVNNVKAGV VNGTGAPGQSPGAGRACESCYTTQSYQWYSWGP PNMQC RLCASCWTYWKKYGGLKMPTRLDGERPGPNRSNMSPHG
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab91987 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    SDS-PAGE
    MS
  • Application notesMS: Use at an assay dependent dilution.
    SDS-PAGE: Use at an assay dependent dilution.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Protein info

    • Alternative names
        Metastasis associated 1Metastasis associated gene 1Metastasis associated gene 1 protein
        Metastasis associated proteinMetastasis associated protein MTA 1Metastasis associated protein MTA1Metastasis-associated protein MTA1MTA 1MTA1MTA1_HUMAN
      see all
  • FunctionMay be involved in the regulation of gene expression by covalent modification of histone proteins. Isoform Long is a corepressor of estrogen receptor (ER). Isoform Short binds to ER and sequesters it in the cytoplasm and enhances non-genomic responses of ER.
  • Tissue specificityWidely expressed. High expression in brain, ovaries, adrenal glands and virgin mammary glands. Higher in tumors than in adjacent normal tissue from the same individual.
  • Sequence similaritiesContains 1 BAH domain.
    Contains 1 ELM2 domain.
    Contains 1 GATA-type zinc finger.
    Contains 1 SANT domain.
  • Developmental stageHighly expressed in metastatic cells.
  • DomainIsoform Short contains a Leu-Arg-Ile-Leu-Leu motif (ER binding motif).
  • Cellular localizationCytoplasm and Nucleus.

    • Target information above from: UniProt accession Q13330 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    MTA1 protein (Tagged-His Tag) images

    • SDS-PAGE - MTA1 protein (Tagged-His Tag) (ab91987)
      SDS-PAGE - MTA1 protein (Tagged-His Tag) (ab91987)
      The image shows an electrophoretic assay performed using an Agilent 5100 ALP. In some images coloured control bands can be seen at 15 kDa (green) and/or 240 kDa (purple). The protein-specific band is blue.

    References for MTA1 protein (Tagged-His Tag) (ab91987)

    ab91987 has not yet been referenced specifically in any publications.

    Publishing research using ab91987 ? Please let us know so that we can cite the reference in this datasheet

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