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Immunocytochemistry/ Immunofluorescence - MTCO1 antibody [1D6] - Mitochondrial Marker (ab14705)
![Immunocytochemistry/ Immunofluorescence - MTCO1 antibody [1D6] - Mitochondrial Marker (ab14705)](/ps/datasheet/images/14/ab14705/MTCO1-Primary-antibodies-ab14705-2.jpg)
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Immunocytochemistry/ Immunofluorescence - MTCO1 antibody [1D6] - Mitochondrial Marker (ab14705)
![Immunocytochemistry/ Immunofluorescence - MTCO1 antibody [1D6] - Mitochondrial Marker (ab14705)](/ps/datasheet/images/14/ab14705/MTCO1-Primary-antibodies-ab14705-4.jpg)
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Product Name
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MTCO1 antibody [1D6] - Mitochondrial Marker
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See all MTCO1 antibodies (3)...
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Product type
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Primary antibodies
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Description
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Mouse monoclonal [1D6] to MTCO1 - Mitochondrial Marker
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Immunogen
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Purified mitochondrial Complex IV subunit I (Human).
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Reacts with
(species key)
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Hu, Ms, Rat, Cow
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Tested applications
(see key)
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ELISA, Flow Cyt, ICC/IF, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: Use at an assay dependent dilution.
Flow Cyt: Use at an assay dependent dilution.
ICC: Use at a concentration of 5 µg/ml.
ICC/IF: Use at an assay dependent dilution (PMID 19200260).
WB: Use at a concentration of 0.5 µg/ml. Detects a band of approximately 40 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Human heart mitochondria.
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Cellular localization
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Mitochondrial Inner membrane
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Research areas
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Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Integration of energy metabolism
Signal Transduction >> Metabolism >> Mitochondrial
Tags & Cell Markers >> Subcellular Markers >> Organelles >> Mitochondria
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Relevance
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Cytochrome c oxidase subunit I (COI or MTCO1) is one of three mitochondrial DNA (mtDNA) encoded subunits (MTCO1, MTCO2, MTCO3) of respiratory Complex IV. Complex IV is located within the mitochondrial inner membrane and is the third and final enzyme of the electron transport chain of mitochondrial oxidative phosphorylation. Complex IV is composed of 13 polypeptides. Subunits I, II, and III (MTCO1, MTCO2, MTCO3) are encoded by mtDNA while subunits IV, Va, Vb, VIa, VIb, VIc, VIIa, VIIb, VIIc, and VIII are nuclear encoded. Mammalian MTCO1 has 12 membrane-spanning alpha-helices (I to XII).
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Mouse
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Clonality
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Monoclonal
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Clone number
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1D6
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Isotype
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IgG2a
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Light chain type
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kappa
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Purity
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IgG fraction
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: PBS Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this MTCO1 antibody [1D6] - Mitochondrial Marker is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for MTCO1 antibody [1D6] - Mitochondrial Marker images, references, products related to ab14705 and other tools.
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MTCO1 antibody [1D6] - Mitochondrial Marker images:
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 Immunocytochemistry/ Immunofluorescence - MTCO1 antibody [1D6] - Mitochondrial Marker (ab14705)
ab14705 staining MTCO1 - Mitochondrial Marker in pig retinal pigment epithelial cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.5% TX-100 and blocked with 5% serum for 20 minutes at 25°C. Samples were incubated with primary antibody (1/2000 in 1% goat serum, 0.1% TX100; PBS) for 16 hours at 4°C. An Alexa Fluor®546-conjugated Goat polyclonal to mouse IgG, dilution 1/500, was used as secondary antibody. This image is courtesy of an Abreview submitted by Dr Vladimir Milenkovic
See Abreview
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Immunocytochemistry/ Immunofluorescence - MTCO1 antibody [1D6] - Mitochondrial Marker (ab14705)
ab14705 staining MTCO1 - Mitochondrial Marker in rat cerebellum primary cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.5% TX-100 and blocked with 5% serum for 20 minutes at 25°C. Samples were incubated with primary antibody (1/2000 in 1% goat serum, 0.1% TX100; PBS) for 16 hours at 4°C. An Alexa Fluor®546-conjugated Goat polyclonal to mouse IgG, dilution 1/5000, was used as secondary antibody. This image is courtesy of an Abreview submitted by Dr Vladimir Milenkovic
See Abreview
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References for MTCO1 antibody [1D6] - Mitochondrial Marker (ab14705)
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This product has been used in: (two most recent references)
Iwasaki R et al. Catechin, green tea component, causes caspase-independent necrosis-like cell death in chronic myelogenous leukemia. Cancer Sci 100:349-56 (2009). ICC/IF; Human. PubMed: 19200260
Zech T et al. Accumulation of raft lipids in T-cell plasma membrane domains engaged in TCR signalling. EMBO J 28:466-76 (2009). WB; Human. PubMed: 19177148
See all 29 publication references for this product.
If you publish research using ab14705 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to MTCO1
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MTCO1 antibody [1D6] - Mitochondrial Marker - more information
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MTCO1 antibody [1D6] - Mitochondrial Marker - related products:
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MTCO1 antibody [1D6] - Mitochondrial Marker - other tools:
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Contact Abcam with a Technical Enquiry about MTCO1 antibody [1D6] - Mitochondrial Marker |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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![Immunocytochemistry/ Immunofluorescence - MTCO1 antibody [1D6] - Mitochondrial Marker (ab14705)](#MTCO1-Primary-antibodies-ab14705-2.jpg)
![Immunocytochemistry/ Immunofluorescence - MTCO1 antibody [1D6] - Mitochondrial Marker (ab14705)](#MTCO1-Primary-antibodies-ab14705-4.jpg)
