Anti-MTCO1 antibody [1D6E1A8] - Mitochondrial Marker (ab14705)

Dear Technical Support!

You have been an absolute great help and we greatly appreciate it!

Our customer has found a bit of a confusion as per the following:

Antibody Anti-MTCO1 antibody [1D6E1A8] - Mitochondrial Marker (ab14705 or MS602-IV).

Catalogue number: MS602-IV
Part/Lot # x
Date of order: 31st January 2011

I was just wondering that the concentration of the antibody is given to be 1 mg/ml 0r 1ug/ ul) on the spec sheet which was mailed to us along with the antibody whereas if we look at the tube, the concentration written is 0.2ug/ul.

Please advise!

Looking forward to your response!

In the meantime, I hope you have a great day!

ANSWER:

Thank you for your inquiry.

Concentrations sometimes need to be adapted for certain antibodies. I would therefore like to double check with you whether the customer is indeed referring to a tube purchased in January 2011 directly from Mitosciences and the leaflet sent with that antibody purchased in January 2011? If so, there must have been an error somewhere andI would be pleased to follow this up with my colleagues from Mitosciences.

Ifthe customer is however referring to the concentration stated on the tube purchased in January 2011 and a datasheet from a later time point, there is most likely no error. Indeed,our online datasheet for example reflects always the concnetration of the product in stock. It can therefore be that in some cases this does not correspond to the antibody purchased previously.

I hope this information is helpful. Please do let me know ifthe customer is referring to the datasheet obtained in January 2011.

Thank you for your ocntinued support! I hope you are having a good day as well!

Does kit work with Drosophila? How is the homology of the antibodies used in ab110413 with Drosophila?

ANSWER:

Thank you for your call earlier.

I now had a look at the homology between the immunogens of the antibodies included in the MitoProfile® Total OXPHOS Rodent WB Antibody Cocktail (ab110413) and the respective proteins in Drosophila.

Unfortunately, the homologies are only between 34% and 76%. This is not enough to expect a cross-reaction of the antibody cocktail.

Therefore I cannot recommend ab110413 with drosophila samples.

I am sorry I did not have a positive answer to your question on this occasion and wish you good luck with your research.

phone call: band is at 35kD on mouse brain

ANSWER:

Merci pour votre appel.

Comme discuté au téléphone, je pense que la bande que vous observez est la bonne. Voici le lien pour un de nos marqueurs de taille, ou nous avons comparé sa migration dans des différents tampons. ab115832 http://www.abcam.com/index.html?datasheet=115832 (or use the following: http://www.abcam.com/index.html?datasheet=115832).

Si vous voudriez que nous regardons le protocole et le résultat ensemble, aussi avec l'aide de notre laboratoire, veuillez s'il vous plaît nous faire parvenir ces informations. Pour vous faciliter à mettre ces informations ensemble, veuillez trouver ci-joint un questionnaire qui nous permet de regrouper le plus d'informations possible sur le protocole que vous avez utilisé avec cet anticorps.
Celui-ci nous permettra de vous fournir la meilleure assistance technique.

Je peux aussi vous rassurer que nous garantissonstous nos produits de fonctionner tel que décrit sur la fiche technique. http://www.abcam.com/abpromise.

J'espère que ces informations vous sont utiles. N'hésitez pas à nous recontacter si vous souhaitez de l'assistance ou si vous avez des autres questions.

Please could you tell mewhat your mitochondrial antibody-MTC02 labels in mitochondria, we are interested in using amitochondrial membrane marker.

ANSWER:

Thank you for contacting Abcam.

For ab3298 (Mitochondria antibody [MTC02), the immunogen was semi purified mitochondrial preparation andthe antibody recognizes a 60 kDa non glycosylated protein component of mitochondria found in human cell.

Another mitochondrial marker you could use is ab14705 (MTCO1 antibody [1D6E1A8), the immunogen for this antibody was purified mitochondrial Complex IV subunit I and this antibody labels the mitochondrion inner membrane.

If there is anything else I can help you with, please let me know.

Please suggest fluoresent conjugated secondaries for:
ab48394
ab14705
ab24170
ab11420
ab2861
Using in IHC-Fr on confocal microscopy, mouse samples.
Suggested positive controls

ANSWER:

Thank you for your telephone call yesterday afternoon.

I am pleased to provide some information regarding suitable secondaries for the primary antibodies you have selected:

ab48394 LC3B antibody, ab24170 LAMP1antibody andab11420 ERp29 antibody are all rabbit polyclonal. Polyclonal antibodies contain mainly IgG isotypes, soyou need toselect an anti Rabbit IgG tested in IHC-Fr. I have selected some FITC conjugated anti rabbit IgG antibodies that are tested in IHC-Fr. I can suggest to review the online datasheets for further information. Please check that your confocal microscope will detect FITC. Also, if you require any different conjugates, please let me know and I can provide a further list for you as we have many available.

ab6717 Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (FITC)
Tested in: Flow Cyt, IHC-P, ELISA, IM, ICC/IF, IHC-Fr
http://www.abcam.com/index.html?datasheet=6717 (or use the following: http://www.abcam.com/index.html?datasheet=6717).

ab7086 Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (FITC), pre-adsorbed
Tested in: Flow Cyt, IHC-P, ELISA, IM, ICC/IF, IHC-Fr
http://www.abcam.com/index.html?datasheet=7086 (or use the following: http://www.abcam.com/index.html?datasheet=7086).


The ab14705 MTCO1 antibody [1D6E1A8] and ab2861 SERCA2 ATPase antibody [2A7-A1] are both mouse monoclonal IgG2a.

We have no anti mouse IgG2a secondaries tested in IHC-Fr, but in this case you canuse a general anti-mouse IgG.I have selected an FITC conjugated secondary, but again please let me know if you would prefer other conjugates as we have many available.

ab6785 Goat polyclonal Secondary Antibody to Mouse IgG - H&L (FITC)
Tested in: IHC (PFA fixed), Flow Cyt, IHC-P, WB, ELISA, IM, ICC/IF, IHC-Fr
http://www.abcam.com/index.html?datasheet=6785 (or use the following: http://www.abcam.com/index.html?datasheet=6785).

Both ab2861 and ab14705 are mouse antibodies. These are tested successfully in mouse, and so I would like to reassure you we can provide our guarantee for the results. However, because you are staining mouse tissue, I can suggest it may be better to select primary antibodies that are not raised in mouse. When you add the anti-mouse secondary during the staining, it is possible this can bind to mouse antibodies within the tissue and give some background staining.

You may like to consider the following instead:
ab3625 SERCA2 ATPase antibody
Rabbit polyclonal
Tested in: IHC-P, IP, WB, ICC/IF, IHC-Fr
Reacts with: Hu, Ms, Rat, Pig, Rb

I am sorry there are no alternatives for the MTCO1.

If you require some further information regarding antibodies and IHC, as discussed on the telephone I am including some links from our website. These can be found from the scientific support tab on the homepage:

Antibody guide:
http://www.abcam.com/index.html?pageconfig=resource&rid=11287

Protocols (Select Immunostaining from the list on the following protocolspage):
http://www.abcam.com/index.html?pageconfig=popular_protocols

Fluorochrome guides:
http://docs.abcam.com/pdf/immunology/fluorochrome_guide.pdf
http://www.abcam.com/index.html?pageconfig=resource&rid=11755

How to choose the right secondary:
http://www.abcam.com/index.html?pageconfig=resource&rid=12825

How to choose a conjugate:
http://www.abcam.com/index.html?pageconfig=resource&rid=12922

And the following external website may also be helpful:
http://www.ihcworld.com/

I hope this will be helpful. If you require any further information or assistance, please do not hesitate to contact us (mailto:technical@abcam.com).