Anti-MTHFD1 antibody (ab70203)
Key features and details
- Mouse polyclonal to MTHFD1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-MTHFD1 antibody
See all MTHFD1 primary antibodies -
Description
Mouse polyclonal to MTHFD1 -
Host species
Mouse -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein within Human MTHFD1. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
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Positive control
- MTHFD1 transfected 293T cell lysate, human placenta cell lysate
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Constituent: 100% PBS -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab70203 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/1000. Detects a band of approximately 95 kDa (predicted molecular weight: 102 kDa).
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Notes |
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WB
1/500 - 1/1000. Detects a band of approximately 95 kDa (predicted molecular weight: 102 kDa). |
Target
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Tissue specificity
Ubiquitous. -
Pathway
One-carbon metabolism; tetrahydrofolate interconversion. -
Involvement in disease
Defects in MTHFD1 may be a cause of susceptibility to folate-sensitive neural tube defects (folate-sensitive NTD) [MIM:601634]. The most common NTDs are open spina bifida (myelomeningocele) and anencephaly. Genetic defects in MTHFD1 may affect the risk of spina bifida via the maternal rather than the embryonic genotype.
Genetic variation in MTHFD1 could be associated with susceptibility to colorectal cancer (CRC) [MIM:114500]. -
Sequence similarities
In the N-terminal section; belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family.
In the C-terminal section; belongs to the formate--tetrahydrofolate ligase family. -
Domain
This trifunctional enzyme consists of two major domains: an N-terminal part containing the methylene-THF dehydrogenase and cyclohydrolase activities and a larger C-terminal part containing formyl-THF synthetase activity. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 4522 Human
- Omim: 172460 Human
- SwissProt: P11586 Human
- Unigene: 652308 Human
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Alternative names
- 5,10 methylenetetrahydrofolate dehydrogenase 5,10 methylenetetrahydrofolate cyclohydrolase,10 formyltetrahydrofolate synthetase antibody
- C 1 tetrahydrofolate synthase cytoplasmic antibody
- C1 tetrahydrofolate synthase cytoplasmic antibody
see all
Images
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Anti-MTHFD1 antibody (ab70203) at 1/500 dilution + human placenta at 25 µg
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size: 102 kDa
Observed band size: 95 kDa why is the actual band size different from the predicted? -
All lanes : Anti-MTHFD1 antibody (ab70203) at 1/500 dilution
Lane 1 : MTHFD1 transfected 293T cell lysate
Lane 2 : non transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size: 102 kDa
Observed band size: 95 kDa why is the actual band size different from the predicted?
Datasheets and documents
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Datasheet download
References (6)
ab70203 has been referenced in 6 publications.
- Tanaka K et al. Glioma cells require one-carbon metabolism to survive glutamine starvation. Acta Neuropathol Commun 9:16 (2021). PubMed: 33468252
- Di Marcantonio D et al. ATF3 coordinates serine and nucleotide metabolism to drive cell cycle progression in acute myeloid leukemia. Mol Cell 81:2752-2764.e6 (2021). PubMed: 34081901
- Sdelci S et al. MTHFD1 interaction with BRD4 links folate metabolism to transcriptional regulation. Nat Genet 51:990-998 (2019). PubMed: 31133746
- Yu H et al. Overexpression of MTHFD1 in hepatocellular carcinoma predicts poorer survival and recurrence. Future Oncol 15:1771-1780 (2019). PubMed: 30997850
- Zheng Y et al. Mitochondrial One-Carbon Pathway Supports Cytosolic Folate Integrity in Cancer Cells. Cell 175:1546-1560.e17 (2018). PubMed: 30500537
- Ducker GS et al. Human SHMT inhibitors reveal defective glycine import as a targetable metabolic vulnerability of diffuse large B-cell lymphoma. Proc Natl Acad Sci U S A 114:11404-11409 (2017). PubMed: 29073064