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Western blot - MTRF1 antibody (ab69898)
(enlarge)
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Western blot - MTRF1 antibody (ab69898)
(enlarge)
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Product Name
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MTRF1 antibody
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Product type
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Primary antibodies
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Description
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Mouse polyclonal to MTRF1
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Immunogen
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MTRF1 (ENSP00000239852) human protein.
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Reacts with
(species key)
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Hu
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Tested applications
(see key)
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WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
WB: 1/500 - 1/1000. Detects a band of approximately 50 kDa (predicted molecular weight: 52 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Human liver; MTRF1 transfected 293T cell lysate
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Cellular localization
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Mitochondrial
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Research areas
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Chromatin and Nuclear Signaling >> DNA / RNA >> Translation >> Regulation
Chromatin and Nuclear Signaling >> DNA / RNA >> Translation >> Mito. Translation
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Relevance
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Mitochondrial peptide chain release factor that directs the termination of translation in response to the peptide chain non cognate termination stop codons AGG and AGA.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Mouse
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Protein G purified
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Storage buffer
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Preservative: None
Constituents: 1X PBS, pH 7.2
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Form
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Liquid
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Concentration
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0.500 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this MTRF1 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for MTRF1 antibody images, references, products related to ab69898 and other tools.
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MTRF1 antibody images:
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 Western blot - MTRF1 antibody (ab69898)
MTRF1 antibody (ab69898) at 1/500 dilution + human liver at 25 µg
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size : 52 kDa
Observed band size : 50 kDa (why is the actual band size different from the predicted?)
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Western blot - MTRF1 antibody (ab69898)
All lanes : MTRF1 antibody (ab69898) at 1/500 dilution
Lane 1 : MTRF1 transfected 293T cell lysate
Lane 2 : non transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size : 52 kDa
Observed band size : 18 kDa (why is the actual band size different from the predicted?)
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Search PubMed (MEDLINE) for references to MTRF1
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MTRF1 antibody - more information
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Customer reviews (feedback) regarding MTRF1 antibody |
Customer FAQs regarding MTRF1 antibody |
Protocols for MTRF1 antibody |
Price and availability of products related to MTRF1 antibody |
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MTRF1 antibody - related products:
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MTRF1 antibody - other tools:
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Contact Abcam with a Technical Enquiry about MTRF1 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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