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Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> Translation >> Mito. Translation
Overview
Product name
Anti-MTRF1 antibody
Description
Mouse polyclonal to MTRF1
Tested applications
Cross reactivity
Reacts with
Human
Immunogen
MTRF1 (ENSP00000239852) human protein.
Positive control
Human liver; MTRF1 transfected 293T cell lysate
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 1X PBS, pH 7.2
Concentration
Concentration information loading...
Purity
Protein G purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> DNA / RNA >> Translation >> Regulation
Epigenetics and Nuclear Signaling >> DNA / RNA >> Translation >> Mito. Translation
Applications
Show applications key
Our Abpromise guarantee covers the use of ab69898 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/500 - 1/1000.Detects a band of approximately 50 kDa (predicted molecular weight: 52 kDa).
Target
Relevance
Mitochondrial peptide chain release factor that directs the termination of translation in response to the peptide chain non cognate termination stop codons AGG and AGA.
Cellular localization
Mitochondrial
Alternative names
- MRF1 antibody
- Peptide chain release factor 1, mitochondrial antibody
Database links
- Entrez Gene: 9617 Human
- Omim: 604601 Human
- SwissProt: O75570 Human
Anti-MTRF1 antibody images:
Western blot - MTRF1 antibody (ab69898)
Anti-MTRF1 antibody (ab69898) at 1/500 dilution + human liver at 25 µg
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size : 52 kDa
Observed band size : 50 kDa (why is the actual band size different from the predicted?)
Western blot - MTRF1 antibody (ab69898)
All lanes : Anti-MTRF1 antibody (ab69898) at 1/500 dilution
Lane 1 : MTRF1 transfected 293T cell lysate
Lane 2 : non transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size : 52 kDa
Observed band size : 18 kDa (why is the actual band size different from the predicted?)
References for Anti-MTRF1 antibody (ab69898)
ab69898 has not yet been referenced specifically in any publications.
Publishing research using ab69898? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"