Anti-MTX1/MTX antibody (ab233205)
Key features and details
- Rabbit polyclonal to MTX1/MTX
- Suitable for: WB, IHC-P
- Reacts with: Rat, Human
Overview
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Product name
Anti-MTX1/MTX antibody
See all MTX1/MTX primary antibodies -
Description
Rabbit polyclonal to MTX1/MTX -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Rat, Human
Predicted to work with: Mouse, Cow, Pig, Cynomolgus monkey -
Immunogen
Recombinant fragment (His-T7-tag) corresponding to Human MTX1/MTX aa 150-400. Expressed in E.coli.
Database link: Q13505 -
Positive control
- WB: Recombinant human MTX1/MTX protein; Jurkat and MCF7 cell lysates; Rat cerebrum lysate. IHC-P: Human stomach and kidney tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.011% Proclin 300
Constituents: 55.77% Glycerol (glycerin, glycerine), 44.219% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Antigen-specific affinity chromatography followed by Protein A affinity chromatography. -
Clonality
Polyclonal -
Research areas
Associated products
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Compatible Secondaries
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab233205 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 0.2 - 2 µg/ml. Predicted molecular weight: 51 kDa.
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IHC-P |
Use a concentration of 5 - 20 µg/ml.
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Notes |
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WB
Use a concentration of 0.2 - 2 µg/ml. Predicted molecular weight: 51 kDa. |
IHC-P
Use a concentration of 5 - 20 µg/ml. |
Target
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Function
Involved in transport of proteins into the mitochondrion. Essential for embryonic development. -
Sequence similarities
Belongs to the metaxin family. -
Post-translational
modificationsUbiquitinated by PARK2 during mitophagy, leading to its degradation and enhancement of mitophagy. Deubiquitinated by USP30. -
Cellular localization
Membrane. Mitochondrion outer membrane. - Information by UniProt
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Database links
- Entrez Gene: 664655 Cow
- Entrez Gene: 101926624 Cynomolgus monkey
- Entrez Gene: 4580 Human
- Entrez Gene: 17827 Mouse
- Entrez Gene: 664654 Pig
- Omim: 600605 Human
- SwissProt: Q2TBS1 Cow
- SwissProt: Q4R3I0 Cynomolgus monkey
see all -
Alternative names
- Metaxin 1 antibody
- Metaxin-1 antibody
- Mitochondrial outer membrane import complex protein 1 antibody
see all
Images
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Anti-MTX1/MTX antibody (ab233205) at 2 µg/ml + Recombinant human MTX1/MTX protein
Predicted band size: 51 kDa -
Anti-MTX1/MTX antibody (ab233205) at 2 µg/ml + Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate
Predicted band size: 51 kDa -
Anti-MTX1/MTX antibody (ab233205) at 2 µg + Rat cerebrum lysate
Predicted band size: 51 kDa -
Anti-MTX1/MTX antibody (ab233205) at 2 µg/ml + MCF7 (human breast adenocarcinoma cell line) cell lysate
Predicted band size: 51 kDa -
Formalin-fixed, paraffin-embedded human kidney tissue stained for MTX1/MTX using ab233205 at 20 μg/ml in immunohistochemical analysis. DAB staining.
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Formalin-fixed, paraffin-embedded human stomach tissue stained for MTX1/MTX using ab233205 at 20 μg/ml in immunohistochemical analysis. DAB staining.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab233205 has been referenced in 1 publication.
- Lu X et al. Ultrastructural and proteomic profiling of mitochondria-associated endoplasmic reticulum membranes reveal aging signatures in striated muscle. Cell Death Dis 13:296 (2022). PubMed: 35368021