MUC1 antibody [115D8] (ab36690)

Product Name 

MUC1 antibody [115D8]

Product type 

Primary antibodies

Description 

Mouse monoclonal [115D8] to MUC1

Immunogen 

Carcinoma cells. No more information available.

Reacts with 
(species key)

Hu

Specificity 

The antibody reacts very strongly with most carcinomas. It occasionally shows a very weak crossreactivity with sarcomas and leukemias. It does not react with normal intestine or colorectal adenomas, but reacts with colorectal carcinomas.

Tested applications 
(see key)

IHC-Fr, IHC-P, IP, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
IHC-P: 1/10 - 1/20.
IHC-Fr: Use at an assay dependent dilution.
IP: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Apical cell membrane; Single-pass type I membrane protein.

Research areas 

Cancer >> Tumor immunology >> Tumor-associated antigens
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> MUC
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Proteins >> MUC
Tags & Cell Markers >> Cell Type Markers >> Tumor Associated

Relevance 

MUC1 is a large cell surface mucin glycoprotein expressed by most glandular and ductal epithelial cells and some hematopoietic cell lineages. It is expressed on most secretory epithelium, including mammary gland and some hematopoietic cells. It is expressed abundantly in lactating mammary glands and overexpressed abundantly in >90% breast carcinomas and metastases. Transgenic MUC1 has been shown to associate with all four cebB receptors and localize with erbB1 (EGFR) in lactating glands. The MUC1 gene contains seven exons and produces several different alternatively spliced variants. The major expressed form of MUC1 uses all seven exons and is a type 1 transmembrane protein with a large extracellular tandem repeat domain. The tandem repeat domain is highly O glycosylated and alterations in glycosylation have been shown in epithelial cancer cells.

Database links 

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

115D8

Isotype 

IgG2b

Purity 

Protein G purified

Storage buffer 

Preservative: 0.02% Sodium Azide
Constituents: PBS

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

0.050 mg/ml

Storage instructions 

Store at +4°C.

References for MUC1 antibody [115D8] (ab36690)

  This product has been used in: (two most recent references)

Takeuchi H et al. The epitope recognized by the unique anti-MUC1 monoclonal antibody MY.1E12 involves sialyl alpha 2-3galactosyl beta 1-3N-acetylgalactosaminide linked to a distinct threonine residue in the MUC1 tandem repeat. J Immunol Methods 270:199-209 (2002). PubMed: 12379325

Shibuya C et al. Immunohistochemical study of a monoclonal antibody 115D8 against human milk-fat globule membrane (MAM-6) in some histological types of breast cancer. Nippon Geka Hokan 59:295-301 (1990). PubMed: 2130793

See all 4 publication references for this product.


If you publish research using ab36690 please let us know so that we can cite the reference on this datasheet.

Customer reviews (feedback) regarding MUC1 antibody [115D8]

Customer FAQs regarding MUC1 antibody [115D8]

Protocols for MUC1 antibody [115D8]

Price and availability of products related to MUC1 antibody [115D8]

MUC1 antibody [115D8] - related products: