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ab28081 has been referenced in 8 publications.
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Anti-MUC1 antibody [C595 (NCRC48)] (ab28081) + Whole cell lysate prepared from human BT20 cells
Predicted band size : 122 kDa
Observed band size : 170 kDa (why is the actual band size different from the predicted?)
Ab28081 staining human normal lung. Staining is localised to the apical cell membrane.
Left panel: with primary antibody at 1 ug/ml. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 antigen retrieval buffers EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
FITC-conjugated ab28081 staining MUC1 in MCF-7 cells (green). Nuclei are counterstained with DAPI (blue).
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