Anti-MUC1 antibody [C595 (NCRC48)] (ab28081)

does C595 react with murine muc-1?

ANSWER:

Thank you for contacting us.

The epitope has only a 50% homology with the murine muc1. We therefore do not recommend this product for use in murine.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

thank you for the answer. i see that in your catalog you have 4 MUC1 protein. Can you suggest me witch one i 've to select for the antibody that you advise me?? Could you advise me the antigen for the Ab28081?





best regards,

ANSWER:

Thank you for your inquiry.

Unfortunately, we do not offer a MUC1 fulllength protein. The MUC1 protein (ab80082) is only a recombinant fragment and all others are peptides. I am sorry for the inconvenience but I cannot recommend any of these as positive control for your experiment.

The immunogen for ab28081 washuman Urinary MUC-1 mucin.I am happy to confirm that the epitopethis clone ([C595 (NCRC48)])recognizes was mapped to the peptide epitope ARG-PRO-ALA-PRO within the protein core of the mucin. The epitope sequence RPAP is a repeated motif that occurs about 40 times along the length of the protein (between residues 126 to 965).

I am sorry that I could not answer all your questions on this occasion and hope this information was helpful nevertheless.

Dear AbCam,
i would like to perform an ELISA sandwich assay for the determination of MUC1 antigen using this two antibodies:
1) ab28081
2) ab109185
i would like to know if it possible to use these two antibodies together; if these antibodies are not yet tested,
is possible to have a discount or a free sample if i decide to purchase?

ANSWER:

Thank you for your inquiry.

I can confirm that these two antibodies were not tested in sandwich ELISA yet. Unfortunately, we cannot give any discount. But there would be the possiblity to take part in our testing discount programm and to recieve a free antibody.

But I cannot recommend it in this case because we do not even know if ab109185 work in ELISA at all and we do have antibodies that are tested and guaranteed for sandwich ELISA in our catalogue:

ab10119 (Anti-MUC1 antibody [M3A106] (HRP)) can be be paired for ELISA with Mouse monoclonal [M10H6] to MUC1 (ab10117) and Mouse monoclonal [M4H2] to MUC1 (ab10120) and Mouse monoclonal [M8C9] to MUC1 (ab10123).

Please follow these links to the respective datasheets:

Click here (or use the following: http://www.abcam.com/index.html?datasheet=10119).

Click here (or use the following: http://www.abcam.com/index.html?datasheet=10117).

Click here (or use the following: http://www.abcam.com/index.html?datasheet=10120).

Click here (or use the following: http://www.abcam.com/index.html?datasheet=10123).

I hope this information is helpful. Please do not hesiate to conatc me with any further qestions.

Dear Abcam, i would like to perform an ELISA sandwich assay using this antibodies:

1) Anti-MUC1 antibody C595 NCRC48, Mouse monoclonal, (ab28081) 2) Anti-MUC1 antibody, Rabbit polyclonal, (ab15481)

i've to question:

1) Can these antibodies use together? 2) Can you suggest me a compatible MUC1 protein? with my best regards,

ANSWER:

Sorry for the delay in contacting you with regards to this query. We have a number of pairs of mouse monoclonals which can be used to detect the under or unglycosylated form of MUC1 (for example ab10117 which can be paired with HRP conjugated ab24471). However if you are interested in the glycosylated form of MUC1, unfortunately we have not tested any antibody pairs in order to detect this form and therefore cannot guarantee that it will work. However, form the pair which you presented, ab28081 has been tested in ELISA with human protein and should therefore be suitable for this application. However, I cannot be sure that the rabbit polyclonal ab15481 will work as it has not been tested in ELISA before and has only been used in applications where the immunogen may be in the denatured form. Two possible alternative would be ab79226 which recognises the Y1229 phosphorylated form (cytoplasmic) or ab14690 which again recognises the underglycosylated form (extracellular) but have both been tested using ELISA. Alternatively, ab80952 is an Armenian Hamster monoclonal reactive against the cytoplasmic tail of MUC1, or ab37435 which is a rabbit polyclonal, again directed against the cytoplasmic domain. Alternatively ab45167 is a rabbit monoclonal which recognises the extracellular domain but only of isoform 7 or MUC1. These have been used in flow cytometry and immunoprecipitation and are therefore more likely to be able to detect the native form of the protein. If you would like to test ab15481 as you suggested (or any of the other antibodies suggested which have not been used in ELISA yet), as it has not been used in ELISA before I can offer a testing discount. This would involve you purchasing the antibody, using it and letting us know the results via an Abreview (positive or negative), you would then be eligible for a free primary antibody of your choice from our catalog. More information on this offer can be found here: www.abcam.com/collaborationdiscount If you need a secondary antibody for this application we have a very easy to use search engine to allow you to find exactly what you are looking for: http://www.abcam.com/index.html?pageconfig=productmap&cl=918 If you would like any further information, help in choosing a suitable secondary antibody or you are interested in applying for the testing discount please do not hesitate to contact me.