MUC1 antibody [DF3] (ab696)

Product Name 

MUC1 antibody [DF3]

Product type 

Primary antibodies

Description 

Mouse monoclonal [DF3] to MUC1

Immunogen 

BALB/C mice were injected with membrane rich fraction of human metastatic breast carcinoma.

Reacts with 
(species key)

Hu

Specificity 

This antibody is specific to 300 kD DF3 antigen present on the apical borders of secretory mammary epithelial cells. It stains glandular epithelia of breast, colon, kidney, liver, lung, pancreas, salivary gland and skin. In tumor tissues, it stains carcinomas of the breast, gastrointestinal tract, lung, kidney, urinary bladder and ovary.

Tested applications 
(see key)

IHC-Fr, IHC-P, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews (Read all Abreviews)

Application notes 
(see key)

Recommended dilutions
IHC-P: 1/10 - 1/25 in an ABC method. Perform heat mediated antigen retrieval before commencing with IHC staining protocol (10 mM citrate buffer, pH 6.0).We suggest an incubation period of 30-60 minutes at room temperature.
IHC-Fr: 1/10 - 1/25 in an ABC method. We suggest an incubation period of 30-60 minutes at room temperature.
WB: 1/200 (see review).

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

IHC: breast carcinoma
WB: breast tumor cell lysate

Cellular localization 

Apical cell membrane; Single-pass type I membrane protein.

Research areas 

Cancer >> Tumor immunology >> Tumor-associated antigens
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> MUC
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Proteins >> MUC
Tags & Cell Markers >> Cell Type Markers >> Tumor Associated

Relevance 

MUC1 is a large cell surface mucin glycoprotein expressed by most glandular and ductal epithelial cells and some hematopoietic cell lineages. It is expressed on most secretory epithelium, including mammary gland and some hematopoietic cells. It is expressed abundantly in lactating mammary glands and overexpressed abundantly in >90% breast carcinomas and metastases. Transgenic MUC1 has been shown to associate with all four cebB receptors and localize with erbB1 (EGFR) in lactating glands. The MUC1 gene contains seven exons and produces several different alternatively spliced variants. The major expressed form of MUC1 uses all seven exons and is a type 1 transmembrane protein with a large extracellular tandem repeat domain. The tandem repeat domain is highly O glycosylated and alterations in glycosylation have been shown in epithelial cancer cells.

Bcl-2 is encoded by a gene involved in the 14; 18 chromosomal translocation. In lymphoid tissues, this antibody stains B lymphocytes as well as T cells.

Database links 

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

DF3

Isotype 

IgG1

Light chain type 

kappa

Purity 

Ascites

Storage buffer 

Preservative: 0.05% Sodium Azide
Constituents: 1% BSA, Tissue culture supernatant

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

Concentration not determined - why is this?

Storage instructions 

Store at +4°C.

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