Anti-MUC1 antibody [M10G4] (HRP) (ab22753)

Overview

  • Product nameAnti-MUC1 antibody [M10G4] (HRP)
    See all MUC1 primary antibodies
  • Description
    Mouse monoclonal [M10G4] to MUC1 (HRP)
  • ConjugationHRP
  • Specificityab22753 binds with high efficiency to a region, (not specified), of GVTSAPDTRPAPGSTAPPAHGVTSA synthetic peptide, spanning the one repeat of VNTR extracellular portion of MUC1 molecule. It reacts with VNTR5 and VNTR20 recombinant unglycosylated fragments of MUC1 protein and underglycosylated MUC1 prepared from tumor fluids or as a result of chemical treatment of human milk MUC1. Affinity > 1 x 108. ab22753 does not recognise natural MUC1 protein isolated from human milk by affinity purification on carbohydrate epitope specific Mabs. No cross-reactivity with egg white avidin.
  • Tested applicationsSandwich ELISA, ELISA, IHC-Fr, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length protein (Human). A high molecular weight, more than 300 kDa, glycoprotein from human milk-fat globule molecule.

Properties

Applications

Our Abpromise guarantee covers the use of ab22753 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent dilution. Can be paired for Sandwich ELISA with Mouse monoclonal [M10G4] to MUC1 (ab10115). ab22753 can be used for the construction of highly effective sandwich ELISA tests for detection and measurements of unglycosylated tumor-associated MUC1 mucins in human serum. Recommended pairing is Mouse monoclonal [M10G4] to MUC1 (ab10115) as capture and this antibody (ab22753) as conjugate. Can be used as Detection antibody with ab10115.
ELISA Use at an assay dependent dilution. Can be paired for ELISA with Mouse monoclonal [M10G4] to MUC1 (ab10115).
IHC-Fr Use at an assay dependent dilution.
ICC/IF Use at an assay dependent dilution.

Target

  • Information by UniProt
  • Database links
  • Alternative names
    • ADMCKD antibody
    • ADMCKD1 antibody
    • Breast carcinoma associated antigen DF3 antibody
    • Breast carcinoma-associated antigen DF3 antibody
    • CA 15-3 antibody
    • CA15 3 antibody
    • CA15 3 antigen antibody
    • CA15.3 antibody
    • Cancer antigen 15-3 antibody
    • Carcinoma associated mucin antibody
    • Carcinoma-associated mucin antibody
    • CD 227 antibody
    • CD227 antibody
    • DF3 antigen antibody
    • EMA antibody
    • Episialin antibody
    • H23 antigen antibody
    • H23AG antibody
    • KL 6 antibody
    • KL-6 antibody
    • KL6 antibody
    • Krebs von den Lungen-6 antibody
    • MAM 6 antibody
    • MAM6 antibody
    • MCD antibody
    • MCKD antibody
    • MCKD1 antibody
    • Medullary cystic kidney disease 1 (autosomal dominant) antibody
    • Medullary cystic kidney disease, autosomal dominant antibody
    • MUC 1 antibody
    • MUC-1 antibody
    • MUC-1/SEC antibody
    • MUC-1/X antibody
    • MUC1 antibody
    • MUC1-alpha antibody
    • MUC1-beta antibody
    • MUC1-CT antibody
    • MUC1-NT antibody
    • MUC1/ZD antibody
    • MUC1_HUMAN antibody
    • Mucin 1 antibody
    • Mucin 1 transmembrane antibody
    • Mucin 1, cell surface associated antibody
    • Mucin-1 subunit beta antibody
    • Peanut reactive urinary mucin antibody
    • Peanut-reactive urinary mucin antibody
    • PEM antibody
    • PEMT antibody
    • Polymorphic epithelial mucin antibody
    • PUM antibody
    • Tumor associated epithelial membrane antigen antibody
    • Tumor associated epithelial mucin antibody
    • Tumor associated mucin antibody
    • Tumor-associated epithelial membrane antigen antibody
    • Tumor-associated mucin antibody
    see all

References for Anti-MUC1 antibody [M10G4] (HRP) (ab22753)

ab22753 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your patience. I suggest a starting range of 5-10 ug/ml for a direct ELISA. You may be able to use it less dilute depending on your samples. Please let me know if you have additional questions.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"