Anti-MUC1 antibody [M3A106] (HRP) (ab10119)

thank you for the answer. i see that in your catalog you have 4 MUC1 protein. Can you suggest me witch one i 've to select for the antibody that you advise me?? Could you advise me the antigen for the Ab28081?





best regards,

ANSWER:

Thank you for your inquiry.

Unfortunately, we do not offer a MUC1 fulllength protein. The MUC1 protein (ab80082) is only a recombinant fragment and all others are peptides. I am sorry for the inconvenience but I cannot recommend any of these as positive control for your experiment.

The immunogen for ab28081 washuman Urinary MUC-1 mucin.I am happy to confirm that the epitopethis clone ([C595 (NCRC48)])recognizes was mapped to the peptide epitope ARG-PRO-ALA-PRO within the protein core of the mucin. The epitope sequence RPAP is a repeated motif that occurs about 40 times along the length of the protein (between residues 126 to 965).

I am sorry that I could not answer all your questions on this occasion and hope this information was helpful nevertheless.

Dear AbCam,
i would like to perform an ELISA sandwich assay for the determination of MUC1 antigen using this two antibodies:
1) ab28081
2) ab109185
i would like to know if it possible to use these two antibodies together; if these antibodies are not yet tested,
is possible to have a discount or a free sample if i decide to purchase?

ANSWER:

Thank you for your inquiry.

I can confirm that these two antibodies were not tested in sandwich ELISA yet. Unfortunately, we cannot give any discount. But there would be the possiblity to take part in our testing discount programm and to recieve a free antibody.

But I cannot recommend it in this case because we do not even know if ab109185 work in ELISA at all and we do have antibodies that are tested and guaranteed for sandwich ELISA in our catalogue:

ab10119 (Anti-MUC1 antibody [M3A106] (HRP)) can be be paired for ELISA with Mouse monoclonal [M10H6] to MUC1 (ab10117) and Mouse monoclonal [M4H2] to MUC1 (ab10120) and Mouse monoclonal [M8C9] to MUC1 (ab10123).

Please follow these links to the respective datasheets:

Click here (or use the following: http://www.abcam.com/index.html?datasheet=10119).

Click here (or use the following: http://www.abcam.com/index.html?datasheet=10117).

Click here (or use the following: http://www.abcam.com/index.html?datasheet=10120).

Click here (or use the following: http://www.abcam.com/index.html?datasheet=10123).

I hope this information is helpful. Please do not hesiate to conatc me with any further qestions.

I found out that you sell a large number of antibodies against the human cell surface antigen MUC1. Does any of the ones you sell react with the extracellular domain further out than the cleavage site (or with the cleavage site)? Also, do they react with the mature form (several of the muc1 abs only react with low glycosylated precursors)? And thirdly, do they react with rhesus monkey MUC1?

ANSWER:

Thank you for your enquiry regarding our MUC1 antibodies.

There is indeed a large selection of those antibodies available in our catalogue and I have summarised below the information we have regarding the epitope recognised and whether we know if the antibody recognises various glycosylated forms of the protein. The extracellular part of the human protein is located with aa24-1158. Many antibodies recognize The APDTR repeat found in this region as you will see below. None of those antibodies have been tested against Macaca mulatta tissue, and a homology search showed 78% homology between the human protein and the monkey protein which may be low for using monoclonal antibodies.

Ab8323- Binds with high efficiency to epitope within the VNTR tandem repeat peptide region of MUC1 molecule. Recognizes underglycosylated and natural MUC1 protein.

Ab696. epitope not mapped. This antibody is specific to 300 kD DF3 antigen.

Ab14690. Immunogen sequence is unknown (the antibody is not made by Abcam and kept confidential from us). It is a “60 aa long synthetic peptide representing a 20 amino acid repeat sequence from mucin 1". The antibody labels underglycosylated mucin.

15481. Immunogen sequence is unknown (the antibody is not made by Abcam and kept confidential from us). We know the immunogen is from the cytoplasmic tail part of the protein. Type of underglycosylated or mature protein recognized: unknown.

Ab8665: It recognizes the multiple protein epitope that is relatively insensitive to glycosylation. Therefore, it is very sensitive in the detection of episialin expression in different epithelial tissues. Multiple epitopes.

Ab22711. SM3 antibody recognises the under-glycosylated form of MUC1. Epitope not mapped.

Ab8605. epitope not mapped. Type of underglycosylated or mature protein recognized: unknown.

Ab8618. The dominant epitope of this antibody is the 5-mer PDTRP of MUC1 tandem repeat as established with “epitope fingerprinting.” Type of underglycosylated or mature protein recognized: unknown.

Ab8606: The dominant epitope of this antibody is the 12-mer GVTSAPDTRPAP of the MUC1 tandem repeat as established with “epitope fingerprinting”. Type of underglycosylated or mature protein recognized: unknown.

Ab8608. The dominant epitope of this antibody is the 7mer TSAPDTR of the MUC1 tandem repeat as established with epitope fingerprinting. Type of underglycosylated or mature protein recognized: unknown.

Ab8607: The dominant epitope of this antibody is APDTR as established with “epitope fingerprinting.” Type of underglycosylated or mature protein recognized: unknown.

Ab8322, ab10115, ab22754, ab22751. Mabs bind with high efficiency with different regions of VTSAPDTRPAPGSTAPPAHGVTSA synthetic peptide spanning the one repeat of VNTR extracellular portion of MUC1 molecule. They react with VNTR5 and VNTR20 recombinant unglycosylated fragments of MUC1 protein and underglycosylated MUC1 prepared from tumor fluids or as a result of chemical treatment of human milk MUC1. These Mabs do not recognize natural MUC1 protein isolated from human milk by affinity purification on carbohydrate epitope specific Mabs.

Ab10118, ab10119 Mabs bind with high efficiency with different epitopes within the VNTR tandem repeat peptide region of MUC1 molecule, which have different conformations and peptide- carbohydrate compositions. Ab efficiently recognize underglycosylated and natural MUC1 protein isolated from human milk by affinity chromatography.

Ab10120. Mabs bind with high efficiency with different epitopes within the VNTR tandem repeat peptide region of MUC1 molecule, which have different conformations and peptide- carbohydrate compositions. Mab M4H2 reacts with VNTR20 recombinant unglycosylated fragment of MUC1 protein (and very weakly with monomeric MUC1 peptide), with deglycosylated and underglycosylated natural MUC1 (but not with completely glycosylated natural milk MUC1).

Ab10124. epitope: peptide repeat RPAP region. Type of underglycosylated or mature protein recognized: unknown.

Ab10114. epitope: Peptide tandem repeat in MUC1 core. Type of underglycosylated or mature protein recognized: unknown. Ab6261, ab853. EPitope: unknow. This antibody reacts with an antigen of 265-400 kD belonging to a heterogeneous group of heavily glycosylated proteins called human milk fat globule proteins.

I hope this information helps, please do not hesitate to contact us if you need any more advice or information to help you choose the correct antibody suitable for your needs.

Customer wantes to know if the following antibodies bind specifically protein A and whether "regular" protein A was used in purification (and not some sort of varient).

ANSWER:

Thank you for your enquiry. Regarding ab8322, ab8323, ab10114, ab10115, ab10118, ab10119, ab10123, and ab10124, the originator was able to say that the antibodies were purified by chromatography on Protein A Sepharose. Regarding ab8607, the originator said that they used a Protein A column made in-house and that the antibody is not 100% purified. Ab8203 was not Protein A purified. This is all the information that I can give you regarding your enquiry.