Anti-MUC1 antibody [M9E7] (ab8322)

subisotype information for mouse mono IgG?

ANSWER:

Thank you for contacting us.

I heard back from the lab: The isotype of ab8322 is IgG. Unfortunately we have not determined the subisotype more specifically.

I am sorry to not have more helpful information for you. Please do not hesitate to contact us if you need any more advice or information. 

I am writing to you concerning the monoclonal antibody anti MUC1 code number 8322.

Could you let me know its concentration? And, if it is possible, could you tell me what do you mean when you say that it does not recognize natural MUC1 protein? Do you mean that it recognizes tumoural MUC1 protein?

I would be very grateful if you could give me any information regarding this matter.

Thank you in advance and looking forward to hearing from you soon.

ANSWER:

Thank you for your enquiry. I apologise for the delay in obtaining this information for you.

This MUC1 antibody reacts with VNTR20 (400aa) recombinant and synthetic TR (20aa) polypeptides corresponding to a multiple or single repeats from MUC1 human glycoprotein. It is known not to interact clearly with the O-glycosylated natural MUC1 obtained from human milk, and reacts to the same extend with MUC1 protein purified from culture media of human T47D adenocarcinoma cell line. It seems that this antibody is directed to continuous MUC1 polypeptide epitopes without O-linked oligosaccharides present in natural MUC1.

I hope this information is helpful, and I have modified the datasheet to make this clearer. Should you have any further questions, please do not hesitate to contact me again.

I found out that you sell a large number of antibodies against the human cell surface antigen MUC1. Does any of the ones you sell react with the extracellular domain further out than the cleavage site (or with the cleavage site)? Also, do they react with the mature form (several of the muc1 abs only react with low glycosylated precursors)? And thirdly, do they react with rhesus monkey MUC1?

ANSWER:

Thank you for your enquiry regarding our MUC1 antibodies.

There is indeed a large selection of those antibodies available in our catalogue and I have summarised below the information we have regarding the epitope recognised and whether we know if the antibody recognises various glycosylated forms of the protein. The extracellular part of the human protein is located with aa24-1158. Many antibodies recognize The APDTR repeat found in this region as you will see below. None of those antibodies have been tested against Macaca mulatta tissue, and a homology search showed 78% homology between the human protein and the monkey protein which may be low for using monoclonal antibodies.

Ab8323- Binds with high efficiency to epitope within the VNTR tandem repeat peptide region of MUC1 molecule. Recognizes underglycosylated and natural MUC1 protein.

Ab696. epitope not mapped. This antibody is specific to 300 kD DF3 antigen.

Ab14690. Immunogen sequence is unknown (the antibody is not made by Abcam and kept confidential from us). It is a “60 aa long synthetic peptide representing a 20 amino acid repeat sequence from mucin 1". The antibody labels underglycosylated mucin.

15481. Immunogen sequence is unknown (the antibody is not made by Abcam and kept confidential from us). We know the immunogen is from the cytoplasmic tail part of the protein. Type of underglycosylated or mature protein recognized: unknown.

Ab8665: It recognizes the multiple protein epitope that is relatively insensitive to glycosylation. Therefore, it is very sensitive in the detection of episialin expression in different epithelial tissues. Multiple epitopes.

Ab22711. SM3 antibody recognises the under-glycosylated form of MUC1. Epitope not mapped.

Ab8605. epitope not mapped. Type of underglycosylated or mature protein recognized: unknown.

Ab8618. The dominant epitope of this antibody is the 5-mer PDTRP of MUC1 tandem repeat as established with “epitope fingerprinting.” Type of underglycosylated or mature protein recognized: unknown.

Ab8606: The dominant epitope of this antibody is the 12-mer GVTSAPDTRPAP of the MUC1 tandem repeat as established with “epitope fingerprinting”. Type of underglycosylated or mature protein recognized: unknown.

Ab8608. The dominant epitope of this antibody is the 7mer TSAPDTR of the MUC1 tandem repeat as established with epitope fingerprinting. Type of underglycosylated or mature protein recognized: unknown.

Ab8607: The dominant epitope of this antibody is APDTR as established with “epitope fingerprinting.” Type of underglycosylated or mature protein recognized: unknown.

Ab8322, ab10115, ab22754, ab22751. Mabs bind with high efficiency with different regions of VTSAPDTRPAPGSTAPPAHGVTSA synthetic peptide spanning the one repeat of VNTR extracellular portion of MUC1 molecule. They react with VNTR5 and VNTR20 recombinant unglycosylated fragments of MUC1 protein and underglycosylated MUC1 prepared from tumor fluids or as a result of chemical treatment of human milk MUC1. These Mabs do not recognize natural MUC1 protein isolated from human milk by affinity purification on carbohydrate epitope specific Mabs.

Ab10118, ab10119 Mabs bind with high efficiency with different epitopes within the VNTR tandem repeat peptide region of MUC1 molecule, which have different conformations and peptide- carbohydrate compositions. Ab efficiently recognize underglycosylated and natural MUC1 protein isolated from human milk by affinity chromatography.

Ab10120. Mabs bind with high efficiency with different epitopes within the VNTR tandem repeat peptide region of MUC1 molecule, which have different conformations and peptide- carbohydrate compositions. Mab M4H2 reacts with VNTR20 recombinant unglycosylated fragment of MUC1 protein (and very weakly with monomeric MUC1 peptide), with deglycosylated and underglycosylated natural MUC1 (but not with completely glycosylated natural milk MUC1).

Ab10124. epitope: peptide repeat RPAP region. Type of underglycosylated or mature protein recognized: unknown.

Ab10114. epitope: Peptide tandem repeat in MUC1 core. Type of underglycosylated or mature protein recognized: unknown. Ab6261, ab853. EPitope: unknow. This antibody reacts with an antigen of 265-400 kD belonging to a heterogeneous group of heavily glycosylated proteins called human milk fat globule proteins.

I hope this information helps, please do not hesitate to contact us if you need any more advice or information to help you choose the correct antibody suitable for your needs.

Customer wantes to know if the following antibodies bind specifically protein A and whether "regular" protein A was used in purification (and not some sort of varient).

ANSWER:

Thank you for your enquiry. Regarding ab8322, ab8323, ab10114, ab10115, ab10118, ab10119, ab10123, and ab10124, the originator was able to say that the antibodies were purified by chromatography on Protein A Sepharose. Regarding ab8607, the originator said that they used a Protein A column made in-house and that the antibody is not 100% purified. Ab8203 was not Protein A purified. This is all the information that I can give you regarding your enquiry.