MUC1 antibody [M9E7] (ab8322)

Product Name 

MUC1 antibody [M9E7]

Product type 

Primary antibodies

Description 

Mouse monoclonal [M9E7] to MUC1

Immunogen 

High molecular weight (more than 300 kDa) glycoprotein from human milk-fat globule molecule

Reacts with 
(species key)

Hu

Specificity 

Reacts with VNTR5 and VNTR20 recombinant unglycosylated fragments of MUC1 protein and underglycosylated MUC. It is directed to continuous MUC1 polypeptide epitopes without O-linked oligosaccharides present in natural MUC1.

No cross reactivity with egg white avidin.
Affinity = > 1.0 x10⁸

Epitope 

Region of GVTSAPDTRPAPGSTAPPAHGVTSA synthetic peptide spanning the one repeat of VNTR extracellular portion of MUC1 molecule.

Tested applications 
(see key)

ELISA, sELISA

Abreviews 

Customer reviews (see key)

Read about Abreviews (Read all Abreviews)

Application notes 
(see key)

Recommended dilutions
ELISA: Use at an assay dependent dilution.
sELISA: Can be paired for ELISA with Mouse monoclonal [M2C5] to MUC1 (ab8323), Mouse monoclonal [M3A106] to MUC1 (HRP) (ab10119), Mouse monoclonal [M9E7] to MUC1 (HRP) (ab22747) and Mouse monoclonal [M2C5] to MUC1 (HRP) (ab24471).
Use at an assay dependent dilution.

ELISA tests for detection and measurements of unglycosylated and under glycosylated tumor-associated MUC1 mucins in human serum. It may be used as a solid-phase reagent for MUC1 isoforms capture. Recommended detection reagents:
(a) enzyme-conjugated version of this antibody for detection of unglycosylated MUC1 (ab22747), or
(b)enzyme-conjugated ab8323 or ab10119 specific to carbohydrate portion of MUC1 (detection of underglycosylated MUC1).

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Apical cell membrane; Single-pass type I membrane protein.

Research areas 

Cancer >> Tumor immunology >> Tumor-associated antigens
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> MUC
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Proteins >> MUC
Tags & Cell Markers >> Cell Type Markers >> Tumor Associated

Relevance 

MUC1 is a large cell surface mucin glycoprotein expressed by most glandular and ductal epithelial cells and some hematopoietic cell lineages. It is expressed on most secretory epithelium, including mammary gland and some hematopoietic cells. It is expressed abundantly in lactating mammary glands and overexpressed abundantly in >90% breast carcinomas and metastases. Transgenic MUC1 has been shown to associate with all four cebB receptors and localize with erbB1 (EGFR) in lactating glands. The MUC1 gene contains seven exons and produces several different alternatively spliced variants. The major expressed form of MUC1 uses all seven exons and is a type 1 transmembrane protein with a large extracellular tandem repeat domain. The tandem repeat domain is highly O glycosylated and alterations in glycosylation have been shown in epithelial cancer cells.

Detection of un-glycosylated and underglycosylated Mucin-1 Glycoprotein

Database links 

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

M9E7

Myeloma 

x63-Ag8.653

Isotype 

IgG

Purity 

Protein A purified

Storage buffer 

PBS with 0.1% sodium azide, pH 7.4

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

7.200 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

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