If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Publishing research using ab15481? Please let us know so that we can cite the reference in this datasheet
ab15481 has been referenced in 7 publications.
Publishing research using ab15481? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Formalin fixed paraffin embedded breast carcinoma stained with anti-MUC1 antibody, ab15481.
ab15481 at 1/200 staining mouse mammary gland tissue sections by IHC-P. The tissue was paraformaldehyde fixed and blocked with BSA. A heat mediated antigen retrieval step was performed. The antibody was incubated with the tissue for 16 hours and then an Alexa-Fluor 488 conjugated goat anti-rabbit antibody was used as the secondary. MUC1 staining (luminal cells) is shown in green. The nuclei were counterstained with DAPI and staining is shown in blue.
This image is courtesy of an anonymous Abreview
ICC/IF image of ab15481 stained Hek293 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab15481, 1µg/ml) overnight at +4ºC. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
4
Call 01223 696 000 or contact us