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Immunohistochemistry (Formalin-fixed paraffin-embedded sections) - MUC1 antibody (ab15481)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - MUC1 antibody (ab15481)
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Immunocytochemistry/ Immunofluorescence - MUC1 antibody (ab15481)
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Product Name
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MUC1 antibody
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See all MUC1 antibodies (41)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to MUC1
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Immunogen
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Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) (Human) from cytoplasmic tail.
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Reacts with
(species key)
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Hu, Ms
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Tested applications
(see key)
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ICC/IF, IHC-Fr, IHC-P
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ICC/IF: Use at a concentration of 1 µg/ml.
IHC-P: 1/100 (10min at RT). Antigen retrieval is not essential but may optimise staining.
IHC-Fr: 1/200.
Is unsuitable for WB.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Breast carcinoma.
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Cellular localization
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Apical cell membrane; Single-pass type I membrane protein.
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Research areas
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Cancer >> Tumor immunology >> Tumor-associated antigens
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> MUC
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Proteins >> MUC
Tags & Cell Markers >> Cell Type Markers >> Tumor Associated
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Relevance
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MUC1 is a large cell surface mucin glycoprotein expressed by most glandular and ductal epithelial cells and some hematopoietic cell lineages. It is expressed on most secretory epithelium, including mammary gland and some hematopoietic cells. It is expressed abundantly in lactating mammary glands and overexpressed abundantly in >90% breast carcinomas and metastases. Transgenic MUC1 has been shown to associate with all four cebB receptors and localize with erbB1 (EGFR) in lactating glands. The MUC1 gene contains seven exons and produces several different alternatively spliced variants. The major expressed form of MUC1 uses all seven exons and is a type 1 transmembrane protein with a large extracellular tandem repeat domain. The tandem repeat domain is highly O glycosylated and alterations in glycosylation have been shown in epithelial cancer cells.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.1% Sodium Azide
Constituents: 1% BSA, 10mM PBS, pH 7.4 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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0.200 mg/ml
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Storage instructions
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Store at +4°C.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this MUC1 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for MUC1 antibody images, references, products related to ab15481 and other tools.
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MUC1 antibody images:
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 Immunohistochemistry (Formalin-fixed paraffin-embedded sections) - MUC1 antibody (ab15481)
Formalin fixed paraffin embedded breast carcinoma stained with anti-MUC1 antibody, ab15481.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - MUC1 antibody (ab15481)
ab15481 at 1/200 staining mouse mammary gland tissue sections by IHC-P. The tissue was paraformaldehyde fixed and blocked with BSA. A heat mediated antigen retrieval step was performed. The antibody was incubated with the tissue for 16 hours and then an Alexa-Fluor 488 conjugated goat anti-rabbit antibody was used as the secondary. MUC1 staining (luminal cells) is shown in green. The nuclei were counterstained with DAPI and staining is shown in blue. This image is courtesy of an anonymous Abreview
See Abreview
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Immunocytochemistry/ Immunofluorescence - MUC1 antibody (ab15481)
ICC/IF image of ab15481 stained Hek293 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab15481, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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References for MUC1 antibody (ab15481)
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This product has been used in: (two most recent references)
Schmitz JM et al. Gastric Mucus Alterations Associated With Murine Helicobacter Infection. J Histochem Cytochem : (2009). PubMed: 19153195
van Miltenburg MH et al. Complete focal adhesion kinase deficiency in the mammary gland causes ductal dilation and aberrant branching morphogenesis through defects in Rho kinase-dependent cell contractility. FASEB J 23:3482-93 (2009). PubMed: 19584305
See all 5 publication references for this product.
If you publish research using ab15481 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to MUC1
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MUC1 antibody - more information
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Customer reviews (feedback) regarding MUC1 antibody |
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Price and availability of products related to MUC1 antibody |
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MUC1 antibody - related products:
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MUC1 antibody - other tools:
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Contact Abcam with a Technical Enquiry about MUC1 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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