MUC16 antibody [10G12] (ab19551)

Product Name 

MUC16 antibody [10G12]

Product type 

Primary antibodies

Description 

Mouse monoclonal [10G12] to MUC16

Immunogen 

Ovarian Cancer Antigen.

Reacts with 
(species key)

Hu

Specificity 

Recognizes an epitope on the CA125 containing mucin. The antibody does not recognize the OC125 epitope.

Tested applications 
(see key)

ELISA, IHC-Fr, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
ELISA: Use at an assay dependent dilution. IHC-Fr: Use at a concentration of 2 µg/ml. WB: Use at a concentration of 2 µg/ml. Dilution optimised using Chromogenic detection. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Cell membrane; Single pass type I membrane protein. Secreted, extracellular space. Note May be liberated into the extracellular space following the phosphorylation of the intracellular C-terminus which induces the proteolytic cleavage and liberation of the extracellular domain.

Research areas 

Cancer >> Tumor biomarkers >> Tumor antigens
Cancer >> Tumor immunology >> Tumor-associated antigens
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> MUC
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Proteins >> MUC
Tags & Cell Markers >> Cell Type Markers >> Tumor Associated

Relevance 

MUC16 (CA125) is a serum marker that is used routinely in gynecologic practice to monitor patients with ovarian cancer. It is a mullerian duct differentiation antigen that is overexpressed in epithelial ovarian cancer cells and secreted into the blood, although its expression is not entirely confined to ovarian cancer. Most biochemical studies have concluded that MUC16 is a high molecular mass glycoprotein, although estimates of its size range from 200 to 2000 kDa with smaller “subunits” being described by some investigators.

Database links 

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

10G12

Isotype 

IgG1

Purity 

Protein A purified

Storage buffer 

Preservative: 0.1% Sodium Azide
Constituents: PBS, Ascites.

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Purification notes 

>95% pure (SDS-PAGE). Purified by chromatography. Product is 0.2um filtered.

Form 

Liquid

Concentration 

2.560 mg/ml

Storage instructions 

Store at +4°C.

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