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IHC-P - MUC16 antibody [X325]
![IHC-P - MUC16 antibody [X325]](/ps/datasheet/images/ab10033_12.jpg)
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IHC-P - MUC16 antibody [X325]
![IHC-P - MUC16 antibody [X325]](/ps/datasheet/images/ab10033_2.jpg)
(enlarge)
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IHC-P - MUC16 antibody [X325]
![IHC-P - MUC16 antibody [X325]](/ps/datasheet/images/ab10033_3.jpg)
(enlarge)
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Product Name
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MUC16 antibody [X325]
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See all MUC16 antibodies (12)...
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Product type
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Primary antibodies
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Description
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Mouse monoclonal [X325] to MUC16
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Immunogen
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Full length native protein (purified from human ovarian carcinoma).
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Reacts with
(species key)
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Hu
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Specificity
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Epitope specificity group B (ISOBM classification).
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Tested applications
(see key)
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ELISA, IHC-P, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: Use at an assay dependent dilution.
IHC-P: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution. Detects a band of >1000 kDa.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Cell membrane; Single pass type I membrane protein. Secreted, extracellular space. Note May be liberated into the extracellular space following the phosphorylation of the intracellular C-terminus which induces the proteolytic cleavage and liberation of the extracellular domain.
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Research areas
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Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> MUC
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Proteins >> MUC
Tags & Cell Markers >> Cell Type Markers >> Tumor Associated
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Relevance
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MUC16 (CA125) is a serum marker that is used routinely in gynecologic practice to monitor patients with ovarian cancer. It is a mullerian duct differentiation antigen that is overexpressed in epithelial ovarian cancer cells and secreted into the blood, although its expression is not entirely confined to ovarian cancer. Most biochemical studies have concluded that MUC16 is a high molecular mass glycoprotein, although estimates of its size range from 200 to 2000 kDa with smaller “subunits” being described by some investigators.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Mouse
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Clonality
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Monoclonal
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Clone number
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X325
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Myeloma
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Sp2/0
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Isotype
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IgG1
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Purity
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IgG fraction
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Storage buffer
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Preservative: 0.05% Sodium Azide
Constituents: 0.15M Sodium Chloride, 10mM Tris. pH 7.5 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Purification notes
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Purity tested by electrophoresis.
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Form
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Liquid
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Concentration
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2.000 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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Notes
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Concentration varies from lot to lot and can be provided on request.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this MUC16 antibody [X325] is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for MUC16 antibody [X325] images, references, products related to ab10033 and other tools.
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MUC16 antibody [X325] images:
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 IHC-P - MUC16 antibody [X325]
ab10033 staining MUC16 in human lung tissue (alveolocytes type II) by Immunohistochemistry (FFPE-sections).
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IHC-P - MUC16 antibody [X325]
ab10033 staining MUC16 in human benign ovarian tumor tissue (serous papillary cystadenoma) by Immunohistochemistry (FFPE-sections).
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IHC-P - MUC16 antibody [X325]
ab10033 staining MUC16 in human urinary bladder tissue (transition cells cancer) by Immunohistochemistry (FFPE-sections).
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References for MUC16 antibody [X325] (ab10033)
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This product has been used in:
Yue T et al. The prevalence and nature of glycan alterations on specific proteins in pancreatic cancer patients revealed using antibody-lectin sandwich arrays. Mol Cell Proteomics 8:1697-707 (2009). PubMed: 19377061
If you publish research using ab10033 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to MUC16
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MUC16 antibody [X325] - more information
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Customer reviews (feedback) regarding MUC16 antibody [X325] |
Customer FAQs regarding MUC16 antibody [X325] |
Protocols for MUC16 antibody [X325] |
Price and availability of products related to MUC16 antibody [X325] |
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MUC16 antibody [X325] - related products:
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MUC16 antibody [X325] - other tools:
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Contact Abcam with a Technical Enquiry about MUC16 antibody [X325] |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Pricing information loading
![IHC-P - MUC16 antibody [X325]](/index.html?datasheet=10033#ab10033_12.jpg)
![IHC-P - MUC16 antibody [X325]](/index.html?datasheet=10033#ab10033_2.jpg)
![IHC-P - MUC16 antibody [X325]](/index.html?datasheet=10033#ab10033_3.jpg)
