![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC16 antibody [X75] (ab1107) image](#MUC16-Primary-antibodies-ab1107-2.jpg)
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Products:Tags & Cell Markers >> Cell Type Markers >> Tumor Associated
Overview
Product name
Anti-MUC16 antibody [X75]
See all MUC16 products (15) ...
Description
Mouse monoclonal [X75] to MUC16
Specificity
This antibody specifically reacts with human ovarian carcinoma cells.
Tested applications
WB, IHC-P, Flow Cytmore details
Cross reactivity
Reacts with
Human
Immunogen
CA-125 purified from human ovarian carcinoma.
Positive control
human adenocarcinoma or ovarian carcinoma cell lines or tissues
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.1% Sodium Azide
Constituents: PBS, pH 7.2
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Monoclonal
Clone number
X75
Myeloma
unknown
Isotype
IgG1
Light chain type
unknown
Research areas
Cancer >> Tumor immunology >> Tumor-associated antigens
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> MUC
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Proteins >> MUC
Tags & Cell Markers >> Cell Type Markers >> Tumor Associated
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC16 antibody [X75] (ab1107)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC16 antibody [X75] (ab1107) image](/ps/datasheet/images/1/ab1107/MUC16-Primary-antibodies-ab1107-2.jpg)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab1107 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 - 5 µg/ml.
IHC-P: Use a concentration of 1 - 5 µg/ml.
Flow Cyt: Use at an assay dependent dilution.
Target
Function
Thought to provide a protective, lubricating barrier against particles and infectious agents at mucosal surfaces.
Tissue specificity
Expressed in corneal and conjunctival epithelia (at protein level). Overexpressed in ovarian carcinomas and ovarian low malignant potential (LMP) tumors as compared to the expression in normal ovarian tissue and ovarian adenomas.
Sequence similarities
Contains 2 ANK repeats.
Contains 56 SEA domains.
Domain
Composed of three domains, a Ser-, Thr-rich N-terminal domain, a repeated domain containing more than 60 partially conserved tandem repeats of 156 amino acids each (AAs 12061-21862) and a C-terminal transmembrane contain domain with a short cytoplasmic tail.
Post-translational
modifications
Heavily O-glycosylated; expresses both type 1 and type 2 core glycans.
Heavily N-glycosylated; expresses primarily high mannose and complex bisecting type N-linked glycans.
May be phosphorylated. Phosphorylation of the intracellular C-terminal domain may induce proteolytic cleavage and the liberation of the extracellular domain into the extracellular space.
May contain numerous disulfide bridges. Association of several molecules of the secreted form may occur through interchain disulfide bridges providing an extraordinarily large gel-like matrix in the extracellular space or in the lumen of secretory ducts.
Cellular localization
Cell membrane. Secreted > extracellular space. May be liberated into the extracellular space following the phosphorylation of the intracellular C-terminus which induces the proteolytic cleavage and liberation of the extracellular domain.
Target information above from: UniProt accessionQ8WXI7
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- CA 125 antibody
- CA-125 antibody
- CA125 antibody
see all
Database links
- Entrez Gene: 94025 Human
- Omim: 606154 Human
- SwissProt: Q8WXI7 Human
- Unigene: 432676 Human
Anti-MUC16 antibody [X75] images:
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC16 antibody [X75] (ab1107)
IHC image of ab1107 staining in human breast formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab1107, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
References for Anti-MUC16 antibody [X75] (ab1107)
This product has been referenced in:
- Chen SHet al. Mucin 16 is a functional selectin ligand on pancreatic cancer cells. FASEB J : (2011). IP; Human.Read more (PubMed: 22159147) »
- Heinzelmann-Schwarz VAet al. Overexpression of the cell adhesion molecules DDR1, Claudin 3, and Ep-CAM in metaplastic ovarian epithelium and ovarian cancer. Clin Cancer Res 10:4427-36 (2004).Read more (PubMed: 15240533) »
See all 2 publications for this product
Publishing research using ab1107? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"