Anti-MUC5B antibody [6F10-E4] (ab105460)
Key features and details
- Mouse monoclonal [6F10-E4] to MUC5B
- Suitable for: ELISA, WB
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-MUC5B antibody [6F10-E4]
See all MUC5B primary antibodies -
Description
Mouse monoclonal [6F10-E4] to MUC5B -
Host species
Mouse -
Specificity
Non glycosylated domain of mucin MUC5B. -
Tested applications
Suitable for: ELISA, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human MUC5B conjugated to keyhole limpet haemocyanin. Synthetic peptide from 4 of the cysteine domains of the tandem repeat region.
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General notes
Detection of respiratory and salivary mucins in clinical and experimental material.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituent: 99.98% PBS -
Concentration information loading...
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Purity
Protein A purified -
Primary antibody notes
Detection of respiratory and salivary mucins in clinical and experimental material. -
Clonality
Monoclonal -
Clone number
6F10-E4 -
Myeloma
Sp2 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab105460 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ELISA |
Use at an assay dependent concentration.
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 596 kDa. PubMed: 14678646
Please consult PubMed ID 14678646 for the recommended electrophoresis conditions. |
Notes |
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ELISA
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. Predicted molecular weight: 596 kDa. PubMed: 14678646 Please consult PubMed ID 14678646 for the recommended electrophoresis conditions. |
Target
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Function
Gel-forming mucin that is thought to contribute to the lubricating and viscoelastic properties of whole saliva and cervical mucus. -
Tissue specificity
Expressed on surface airway epithelia. Expressed mainly in mucous cells of submucosal glands of airway tissues. Highly expressed in the sublingual gland. Also found in submaxillary glands, endocervix, gall bladder, and pancreas. -
Sequence similarities
Contains 1 CTCK (C-terminal cystine knot-like) domain.
Contains 3 HAT repeats.
Contains 3 TIL (trypsin inhibitory-like) domains.
Contains 3 VWFC domains.
Contains 4 VWFD domains. -
Domain
The cysteine residues in the Cys-rich subdomain repeats are not involved in disulfide bonding. -
Post-translational
modificationsHighly glycosylated. C-, N- and O-glycosylated. C-mannosylated in the Cys-rich subdomains probably on the first Trp residue of the WXXW motif. Highly O-glycosylated in the Ser/Thr-rich tandem repeat (TR) region. The repeat region is about 59% O-glycosylated with a high abundance of NeuAc(2)Hex(1)HexNac1-ol. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 727897 Human
- Omim: 600770 Human
- SwissProt: Q9HC84 Human
- Unigene: 523395 Human
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Alternative names
- 2300002I04Rik antibody
- A130042M24 antibody
- AV085033 antibody
see all
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (5)
ab105460 has been referenced in 5 publications.
- Saitou M et al. Functional Specialization of Human Salivary Glands and Origins of Proteins Intrinsic to Human Saliva. Cell Rep 33:108402 (2020). PubMed: 33207190
- Martinez-Valdebenito C et al. Characterization of Oral Immunity in Cases and Close Household Contacts Exposed to Andes Orthohantavirus (ANDV). Front Cell Infect Microbiol 10:557273 (2020). PubMed: 33224896
- van Leeuwen S et al. Early salivary changes in multiple myeloma patients undergoing autologous HSCT. Oral Dis 24:972-982 (2018). PubMed: 29637662
- Lachowicz-Scroggins ME et al. Abnormalities in MUC5AC and MUC5B Protein in Airway Mucus in Asthma. Am J Respir Crit Care Med 194:1296-1299 (2016). PubMed: 27845589
- Rousseau K et al. New monoclonal antibodies to non-glycosylated domains of the secreted mucins MUC5B and MUC7. Hybrid Hybridomics 22:293-9 (2003). WB, ELISA ; Human . PubMed: 14678646