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- Proteins and Peptides
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Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »This product is covered by the Abpromise guarantee. Our scientific support team are available to answer any questions or queries - fill out an inquiry form for ab4125 for help.
Alternatively, you can search the previous enquiries about this product to see if your query has already been answered.
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The customer wished to know how to check that the band at 37KDa was the suspected target. The customer also wished to be informed whether she could receive a discount for Ab996. I informed the customer that the first unit would not receive a discount but if three or more units of the same product are purchased on the same purchase order then a discount will be available.
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ANSWER: |
As promised, I have contacted previous academic customers that have purchased this product in order to ascertain whether they have been using this product in Western blotting or not. It is now a case of waiting to see whether I receive any responses. I have not forwarded your contact details to our customers as I do not wish customers to feel pressured in any way. Many of the repeat customers for this product are industrially based and it must be appreciated that they have confidentiality issues to abide by. In the mean time, good luck with your research.
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We have purchased the MURF-1 polyclonal fast-track ab4125 antibody and carried out Western blots on rat skeletal muscle. We get one clear band on the Western blot between 50 and 75 kDa but no band at around 40 kDa where MURF-1 should be. We would be grateful for all feedback. |
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ANSWER: |
Thank you for your message below. I checked the sequence of rat MURF1 and it isn't totally identical to the human immunogen sequence we used. Therefore the antibody may yet work in human samples (although we have had not positive feedback on this yet) but not in rat. I am afraid I don't have any further feedback to give. |
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We would like to use the MURF1 antibody in rat. Do you know whether this is feasible? If no tests exists, is it likely to react?
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ANSWER: |
Thank you for your enquiry. All the information that we have on Ab4125 is located on the online datasheet. Ab4125 is expected to cross-react with Human due to sequence homology but has not yet been tested in other species. Ab4125 is a fast track antibody and is not yet fully characterised; it is shown by ELISA to have high titre values against the immunogen and is currently being tested for use in Western blotting. If you have any more questions, please contact us again. |
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
