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Products:Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> Ubiquitin E2s and E3s >> RING Finger E3 Ligase
Overview
Product name
Anti-MURF1 antibody
See all MURF1 products (4) ...
Description
Goat polyclonal to MURF1
Tested applications
IHC-Fr, ELISA, IHC-Pmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Cow, Dog
Immunogen
Synthetic peptide: DYKSSLIQDGNPM-C, corresponding to N terminal amino acids 2-14 of Human MURF1
DYKSSLIQDG NPM-C
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, 5mg/ml Tris, pH 7.3
Concentration
Concentration information loading...
Purity
IgG fraction
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> Ubiquitin & Ubiquitin Like Modifiers >> E3 Ubiquitin Ligases
Cardiovascular >> Heart >> Contractility >> Contractile Proteins >> Troponin
Cardiovascular >> Heart >> Contractility >> Contractile Proteins >> Myosins
Cardiovascular >> Heart >> Hypertrophy >> Other
Epigenetics and Nuclear Signaling >> Cell cycle >> Ubiquitin ligases
Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> Ubiquitin E2s and E3s >> RING Finger E3 Ligase
Applications
Show applications key
Our Abpromise guarantee covers the use of ab4125 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-Fr: 1/500
ELISA
ELISA: 1/32000ELISA: 1/32000
IHC-P: Use a concentration of 1.25 µg/ml
Target
Function
E3 ubiquitin ligase. Regulates proteasomal degradation of cardiac troponin I/TNNI3 and probably of other sarcomeric-associated proteins. May play a role in striated muscle atrophy and hypertrophy by regulating an anti-hypertrophic PKC-mediated signaling pathway. May regulate the organization of myofibrils through TTN in muscle cells.
Tissue specificity
Muscle specific. Selectively expressed in heart and skeletal muscle. Also expressed in the iris.
Pathway
Protein modification; protein ubiquitination.
Sequence similarities
Contains 1 B box-type zinc finger.
Contains 1 COS domain.
Contains 1 RING-type zinc finger.
Developmental stage
Expressed throughout all developmental stages.
Domain
The RING-type zinc finger mediates interaction with SUMO2 and localization to the nucleus. Also required for the E3 ubiquitin ligase activity.
Cellular localization
Cytoplasm. Nucleus. Localizes to the M- and Z-lines in skeletal muscle. Colocalizes with TNNI3 in myocytes.
Target information above from: UniProt accessionQ969Q1
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- E3 ubiquitin-protein ligase TRIM63 antibody
- FLJ32380 antibody
- IRF antibody
see all
Database links
- Entrez Gene: 84676 Human
- NCBI: 19924163 Human
- Omim: 606131 Human
- SwissProt: Q969Q1 Human
- Unigene: 279709 Human
Anti-MURF1 antibody images:
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - MURF1 antibody (ab4125)
ab4125 at 1.25 µg/ml staining human skeletal muscle by IHC-P. Tissue sections were subjected to steamed antigen retrieval with citrate buffer (pH6), AP-staining. Shows strong staining in rare nuclei, and weak staining of muscle fibres in longitudinal section.
Immunohistochemistry (Frozen sections) - MURF1 antibody (ab4125)
ab4125 staining MURF1 in mouse lung tissue section by Immunohistochemistry (Frozen sections). Tissue underwent fixation in paraformaldehyde and blocked in 1%BSA for 10 minutes at 20°C. The primary antibody was diluted 1/200 and incubated with sample for 20 hours at 20°C. A Biotin conjugated rabbit polyclonal to goat IgG, diluted 1/500 was used as secondary.
This image is courtesy of an Abreview submitted by Miss Silke Vorwald
References for Anti-MURF1 antibody (ab4125)
This product has been referenced in:
- Ochala Jet al. Preferential skeletal muscle myosin loss in response to mechanical silencing in a novel rat intensive care unit model: underlying mechanisms. J Physiol 589:2007-26 (2011).Read more (PubMed: 21320889) »
- Salanova Met al. Molecular biomarkers monitoring human skeletal muscle fibres and microvasculature following long-term bed rest with and without countermeasures. J Anat 212:306-18 (2008).Read more (PubMed: 18221329) »
See all 4 publications for this product
Publishing research using ab4125? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"