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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - MURF1 antibody (ab4125)
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Immunohistochemistry (Frozen sections) - MURF1 antibody (ab4125)
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Product Name
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MURF1 antibody
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See all MURF1 antibodies (3)...
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Product type
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Primary antibodies
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Description
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Goat polyclonal to MURF1
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Immunogen
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Synthetic peptide: DYKSSLIQDGNPM-C, corresponding to N terminal amino acids 2-14 of Human MURF1
BLAST 'DYKSSLIQDGNPM-C' with  or 
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Reacts with
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Hu
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Predicted to react
(species key)
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Dog
(Due to sequence homology)
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Tested applications
(see key)
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ELISA, IHC-Fr, IHC-P
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: 1/32000.
IHC-P: Use at a concentration of 1.25 µg/ml.
IHC-Fr: 1/500.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Cytoplasm (By similarity). Nucleus (By similarity). Note=Localizes to the M- and Z-lines in skeletal muscle. Colocalizes with TNNI3 in myocytes. Referenced from Swiss-Prot entry Q969Q1.
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Research areas
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Cardiovascular >> Heart >> Contractility >> Contractile Proteins >> Troponin
Cardiovascular >> Heart >> Contractility >> Contractile Proteins >> Myosins
Cardiovascular >> Heart >> Hypertrophy >> Other
Chromatin and Nuclear Signaling >> Cell cycle >> Ubiquitin ligases
Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> Ubiquitin E2s and E3s >> RING Finger E3 Ligase
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Relevance
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This protein is a member of the RING zinc finger protein family found in striated muscle and iris. The product of this gene is localized to the Z-line and M-line lattices of myofibrils, where titin's N-terminal and C-terminal regions respectively bind to the sarcomere. In vitro binding studies have shown that this protein also binds directly to titin near the region of titin containing kinase activity. Another member of this protein family binds to microtubules. Since these family members can form heterodimers, this suggests that these proteins may serve as a link between titin kinase and microtubule-dependent signal pathways in muscle.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Goat
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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IgG fraction
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Storage buffer
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Tris saline, 0.02% Na azide, pH7.3 with 0.5% BSA Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Purification notes
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Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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Form
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Liquid
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Concentration
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0.500 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this MURF1 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for MURF1 antibody images, references, products related to ab4125 and other tools.
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MURF1 antibody images:
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 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - MURF1 antibody (ab4125)
ab4125 at 1.25 µg/ml staining human skeletal muscle by IHC-P. Tissue sections were subjected to steamed antigen retrieval with citrate buffer (pH6), AP-staining. Shows strong staining in rare nuclei, and weak staining of muscle fibres in longitudinal section.
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Immunohistochemistry (Frozen sections) - MURF1 antibody (ab4125)
ab4125 staining MURF1 in mouse lung tissue section by Immunohistochemistry (Frozen sections). Tissue underwent fixation in paraformaldehyde and blocked in 1%BSA for 10 minutes at 20°C. The primary antibody was diluted 1/200 and incubated with sample for 20 hours at 20°C. A Biotin conjugated rabbit polyclonal to goat IgG, diluted 1/500 was used as secondary. This image is courtesy of an Abreview submitted by Miss Silke Vorwald
See Abreview
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References for MURF1 antibody (ab4125)
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This product has been used in: (two most recent references)
Salanova M et al. Molecular biomarkers monitoring human skeletal muscle fibres and microvasculature following long-term bed rest with and without countermeasures. J Anat 212:306-18 (2008). PubMed: 18221329
de Palma L et al. Ubiquitin ligases MuRF1 and MAFbx in human skeletal muscle atrophy. Joint Bone Spine 75:53-7 (2008). PubMed: 17977773
See all 3 publication references for this product.
If you publish research using ab4125 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to MURF1
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MURF1 antibody - more information
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Customer reviews (feedback) regarding MURF1 antibody |
Customer FAQs regarding MURF1 antibody |
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Price and availability of products related to MURF1 antibody |
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MURF1 antibody - related products:
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MURF1 antibody - other tools:
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Contact Abcam with a Technical Enquiry about MURF1 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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