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Products:Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> Ubiquitin E2s and E3s >> RING Finger E3 Ligase
Overview
Product name
Anti-MURF1 antibody
See all MURF1 products (4) ...
Description
Mouse monoclonal to MURF1
Tested applications
WB, Flow Cyt, IHC-Pmore details
Cross reactivity
Reacts with
Human
Immunogen
Recombinant fragment: DKSTKLVETA IQSLDEPGGA TFLLTAKQLI KSIVEASKGC QLGKTEQGFE NMDFFTLDLE HIADALRAID FGTDEEEEEF IEEEDQEEEE STEGKEEGH, corresponding to amino acids 254-353 of Human MURF1
DKSTKLVETA IQSLDEPGGA TFLLTAKQLI KSIVEASKGC QLGKTEQGFE NMDFFTLDLE HIADALRAID FGTDEEEEEF IEEEDQEEEE STEGKEEGH
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
PBS, pH 7.2
Concentration
Concentration information loading...
Purity
Protein G purified
Clonality
Monoclonal
Isotype
IgG1
Light chain type
kappa
Research areas
Epigenetics and Nuclear Signaling >> Ubiquitin & Ubiquitin Like Modifiers >> E3 Ubiquitin Ligases
Cardiovascular >> Heart >> Contractility >> Contractile Proteins >> Troponin
Cardiovascular >> Heart >> Contractility >> Contractile Proteins >> Myosins
Cardiovascular >> Heart >> Hypertrophy >> Other
Epigenetics and Nuclear Signaling >> Cell cycle >> Ubiquitin ligases
Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> Ubiquitin E2s and E3s >> RING Finger E3 Ligase
Applications
Show applications key
Our Abpromise guarantee covers the use of ab57865 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 - 5 µg/ml.(This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.)
Flow Cyt: Use 1µg for 106 cells.
IHC-P: Use a concentration of 5 µg/mlPerform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Target
Function
E3 ubiquitin ligase. Regulates proteasomal degradation of cardiac troponin I/TNNI3 and probably of other sarcomeric-associated proteins. May play a role in striated muscle atrophy and hypertrophy by regulating an anti-hypertrophic PKC-mediated signaling pathway. May regulate the organization of myofibrils through TTN in muscle cells.
Tissue specificity
Muscle specific. Selectively expressed in heart and skeletal muscle. Also expressed in the iris.
Pathway
Protein modification; protein ubiquitination.
Sequence similarities
Contains 1 B box-type zinc finger.
Contains 1 COS domain.
Contains 1 RING-type zinc finger.
Developmental stage
Expressed throughout all developmental stages.
Domain
The RING-type zinc finger mediates interaction with SUMO2 and localization to the nucleus. Also required for the E3 ubiquitin ligase activity.
Cellular localization
Cytoplasm. Nucleus. Localizes to the M- and Z-lines in skeletal muscle. Colocalizes with TNNI3 in myocytes.
Target information above from: UniProt accessionQ969Q1
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- E3 ubiquitin-protein ligase TRIM63 antibody
- FLJ32380 antibody
- IRF antibody
see all
Database links
- Entrez Gene: 84676 Human
- NCBI: 19924163 Human
- Omim: 606131 Human
- SwissProt: Q969Q1 Human
- Unigene: 279709 Human
Anti-MURF1 antibody images:
Western blot - MURF1 antibody (ab57865)
Western blot against tagged recombinant protein immunogen using ab57865 MURF1 antibody at 1ug/ml. Predicted band size of immunogen is 37 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - MURF1 antibody (ab57865)
IHC image of ab57865 staining in human skeletal muscle formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab57865, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Flow Cytometry - Anti-MURF1 antibody (ab57865)
Overlay histogram showing HeLa cells stained with ab57865 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab57865, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
References for Anti-MURF1 antibody (ab57865)
ab57865 has not yet been referenced specifically in any publications.
Publishing research using ab57865? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"