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Western blot - MURF1 antibody (ab57865)
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Product Name
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MURF1 antibody
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See all MURF1 antibodies (3)...
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Product type
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Primary antibodies
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Description
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Mouse monoclonal to MURF1
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Immunogen
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Recombinant fragment: DKSTKLVETA IQSLDEPGGA TFLLTAKQLI KSIVEASKGC QLGKTEQGFE NMDFFTLDLE HIADALRAID FGTDEEEEEF IEEEDQEEEE STEGKEEGH, corresponding to amino acids 254-353 of Human MURF1
BLAST 'DKSTKLVETA IQSLDEPGGA TFLLTAKQLI KSIVEASKGC QLGKTEQGFE NMDFFTLDLE HIADALRAID FGTDEEEEEF IEEEDQEEEE ' with  or 
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Reacts with
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Hu
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Tested applications
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WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
WB: Use at a concentration of 1-5 µg/ml.
This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Cytoplasm (By similarity). Nucleus (By similarity). Note=Localizes to the M- and Z-lines in skeletal muscle. Colocalizes with TNNI3 in myocytes. Referenced from Swiss-Prot entry Q969Q1.
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Research areas
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Cardiovascular >> Heart >> Contractility >> Contractile Proteins >> Troponin
Cardiovascular >> Heart >> Contractility >> Contractile Proteins >> Myosins
Cardiovascular >> Heart >> Hypertrophy >> Other
Chromatin and Nuclear Signaling >> Cell cycle >> Ubiquitin ligases
Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> Ubiquitin E2s and E3s >> RING Finger E3 Ligase
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Relevance
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This protein is a member of the RING zinc finger protein family found in striated muscle and iris. The product of this gene is localized to the Z-line and M-line lattices of myofibrils, where titin's N-terminal and C-terminal regions respectively bind to the sarcomere. In vitro binding studies have shown that this protein also binds directly to titin near the region of titin containing kinase activity. Another member of this protein family binds to microtubules. Since these family members can form heterodimers, this suggests that these proteins may serve as a link between titin kinase and microtubule-dependent signal pathways in muscle.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Mouse
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Clonality
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Monoclonal
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Isotype
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IgG1
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Light chain type
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kappa
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Purity
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Protein G purified
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Storage buffer
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Preservative: None
PBS, pH 7.2
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Form
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Liquid
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Concentration
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0.500 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this MURF1 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for MURF1 antibody images, references, products related to ab57865 and other tools.
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MURF1 antibody images:
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 Western blot - MURF1 antibody (ab57865)
Western blot against tagged recombinant protein immunogen using ab57865 MURF1 antibody at 1ug/ml. Predicted band size of immunogen is 37 kDa
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Search PubMed (MEDLINE) for references to MURF1
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MURF1 antibody - more information
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Customer reviews (feedback) regarding MURF1 antibody |
Customer FAQs regarding MURF1 antibody |
Protocols for MURF1 antibody |
Price and availability of products related to MURF1 antibody |
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MURF1 antibody - related products:
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MURF1 antibody - other tools:
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Contact Abcam with a Technical Enquiry about MURF1 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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