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Products:Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> Ubiquitin E2s and E3s >> RING Finger E3 Ligase
Overview
Product name
Anti-MURF1 antibody
See all MURF1 products (4) ...
Description
Rabbit polyclonal to MURF1
Tested applications
WB, IHC-Pmore details
Cross reactivity
Reacts with
Mouse, Human
Predicted to work with
Rat, Cow
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 250 - 350 of Human MURF1.
(Peptide available as ab87125.)
Positive control
This antibody gave a positive signal in the following tissue lysates: human skeletal muscle; mouse skeletal muscle; mouse heart.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> Ubiquitin & Ubiquitin Like Modifiers >> E3 Ubiquitin Ligases
Cardiovascular >> Heart >> Contractility >> Contractile Proteins >> Troponin
Cardiovascular >> Heart >> Contractility >> Contractile Proteins >> Myosins
Cardiovascular >> Heart >> Hypertrophy >> Other
Epigenetics and Nuclear Signaling >> Cell cycle >> Ubiquitin ligases
Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> Ubiquitin E2s and E3s >> RING Finger E3 Ligase
Applications
Show applications key
Our Abpromise guarantee covers the use of ab77577 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 µg/mlDetects a band of approximately 40 kDa (predicted molecular weight: 40 kDa).
IHC-P: Use a concentration of 5 µg/ml
Target
Function
E3 ubiquitin ligase. Regulates proteasomal degradation of cardiac troponin I/TNNI3 and probably of other sarcomeric-associated proteins. May play a role in striated muscle atrophy and hypertrophy by regulating an anti-hypertrophic PKC-mediated signaling pathway. May regulate the organization of myofibrils through TTN in muscle cells.
Tissue specificity
Muscle specific. Selectively expressed in heart and skeletal muscle. Also expressed in the iris.
Pathway
Protein modification; protein ubiquitination.
Sequence similarities
Contains 1 B box-type zinc finger.
Contains 1 COS domain.
Contains 1 RING-type zinc finger.
Developmental stage
Expressed throughout all developmental stages.
Domain
The RING-type zinc finger mediates interaction with SUMO2 and localization to the nucleus. Also required for the E3 ubiquitin ligase activity.
Cellular localization
Cytoplasm. Nucleus. Localizes to the M- and Z-lines in skeletal muscle. Colocalizes with TNNI3 in myocytes.
Target information above from: UniProt accessionQ969Q1
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- E3 ubiquitin-protein ligase TRIM63 antibody
- FLJ32380 antibody
- IRF antibody
see all
Database links
- Entrez Gene: 84676 Human
- Entrez Gene: 140939 Rat
- NCBI: 19924163 Human
- Omim: 606131 Human
- SwissProt: Q969Q1 Human
- SwissProt: Q38HM4 Mouse
- SwissProt: Q91Z63 Rat
- Unigene: 279709 Human
see all
Anti-MURF1 antibody images:
Western blot - MURF1 antibody (ab77577)
All lanes : Anti-MURF1 antibody (ab77577) at 1 µg/ml
Lane 1 : Skeletal Muscle (Human) Tissue Lysate - adult normal tissue (ab29330)
Lane 2 : Heart (Mouse) Tissue Lysate
Lane 3 : Skeletal Muscle (Mouse) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 40 kDa
Observed band size : 40 kDa
Additional bands at : 110 kDa,30 kDa,55 kDa. We are unsure as to the identity of these extra bands.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - MURF1 antibody (ab77577)
IHC image of ab77577 staining in Mouse Skeletal Muscle formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab77577, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
References for Anti-MURF1 antibody (ab77577)
ab77577 has not yet been referenced specifically in any publications.
Publishing research using ab77577? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"